Arabpour Maedeh, Mehrpour Layeghi Sepideh, Majidzadeh-A Keivan, Tavakkoly Bazzaz Javad, Mamivand Ali, Naghizadeh Mohammad Mehdi, Shakoori Abbas
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Genetics Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran.
Biochem Biophys Rep. 2023 Aug 21;35:101531. doi: 10.1016/j.bbrep.2023.101531. eCollection 2023 Sep.
Luminal A and B subtypes of breast cancer (BC) comprises up to 70% of all BC patients. LncRNAs can affect many biological and pathological processes, and dysregulation of them is related to human cancers. The potential role of lncRNA LINC00968 in luminal BC is still unclear.
We analyzed the LINC00968 expression across 44 paired luminal BC tissues from the TCGA-BRCA RNA sequencing dataset. Besides, we used the GEPIA2 web server and GENEVESTIGATOR software, as well. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) assay was performed to confirm the LINC00968 expression in 71 paired luminal BC tissues and two luminal A cell lines (MCF7 and T47D). Moreover, to better understanding the potential role of LINC00968 in luminal BC, computational data analyses including co-expression analysis, functional annotation analysis, and genetic alteration analysis have been done.
The results of data analyses retrieved from BRCA dataset and databases revealed the significant downregulation of LINC00968 in luminal A and B BC. Also, the results of qRT-PCR in luminal BC tissues and cell lines confirmed the earlier data. LINC00968 expression was negatively associated with tumor stage and lymph node metastasis. Additionally, functional annotation analyses revealed that LINC00968 might be involved in vascular development and angiogenesis, extracellular matrix organization, and cell motility and migration. LINC00968 might play role in some cancer-related signaling pathways.
Our study found that downregulation of LINC00968 might promote tumorigenesis, invasion, and metastasis of luminal BC.
乳腺癌（BC）的管腔A型和B型亚型占所有BC患者的70%。长链非编码RNA（lncRNAs）可影响许多生物学和病理过程，其失调与人类癌症相关。lncRNA LINC00968在管腔型BC中的潜在作用仍不清楚。
我们分析了来自TCGA-BRCA RNA测序数据集的44对管腔型BC组织中LINC00968的表达。此外，我们还使用了GEPIA2网络服务器和GENEVESTIGATOR软件。进行实时定量逆转录PCR（qRT-PCR）检测以确认71对管腔型BC组织和两种管腔A型细胞系（MCF7和T47D）中LINC00968的表达。此外，为了更好地了解LINC00968在管腔型BC中的潜在作用，已进行了包括共表达分析、功能注释分析和基因改变分析在内的计算数据分析。
从BRCA数据集和数据库检索到的数据分析结果显示，LINC00968在管腔A型和B型BC中显著下调。此外，管腔型BC组织和细胞系中的qRT-PCR结果证实了早期数据。LINC00968表达与肿瘤分期和淋巴结转移呈负相关。此外，功能注释分析表明，LINC00968可能参与血管发育和血管生成、细胞外基质组织以及细胞运动和迁移。LINC00968可能在一些癌症相关信号通路中发挥作用。
我们的研究发现，LINC00968的下调可能促进管腔型BC的肿瘤发生、侵袭和转移。
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