Mann Navjot Singh, Jhamb Ashu, Kaur Nimrat, Mann Navneet
Department of Conservative Dentistry and Endodontics, National Dental College, Derabassi, Mohali, Punjab, India.
Bioinformation. 2022 Oct 31;18(10):853-857. doi: 10.6026/97320630018853. eCollection 2022.
It is of interest to synthesize Nano eggshell-titanium-dioxide (EB@TiO2) biocomposite and to evaluate its effectiveness in occluding opened dentine tubules. EB@TiO2 was synthesized and characterized using X-ray diffraction (XRD), and Transmission Electron Microscope (TEM). Sixteen simulated bovine dentine discs were prepared and randomly assigned into four groups according to the following treatment (n = 4): Group 1: No treatment; Group 2: eggshell powder; Group 3: EB@TiO2; and Group 4: GIC mousse. These were then, agitated in a solution of 1g powder and 40mL water for 3hours. Thereafter, each dentine discs from the respective groups were post-treated for 5 min with 2wt% citric acid to test their acid resistant characteristics. Scanning Electron Microscope (SEM) was used to observe the effectiveness of occluded dentine pre-treatment and post-treatment. The cytotoxicity of the synthesized EB@TiO2 was tested using NIH 3T3 assay. ANOVA was used to evaluate the mean values of the occluded area ratio and the data of MTS assay. This was followed by a multi-comparison test with Bonferroni correction (α = .05). The XRD confirmed that EB@TiO2 was successfully modified through ball-milling. The TEM revealed the presence of both spherical and irregular particle shape powders. The SEM result showed that EB@TiO2 could effectively occlude open dentine tubules. Equally, the result demonstrated that EB@TiO2 exhibited the highest acid resistant stability post-treatment. NIH 3T3 assay identified that EB@TiO2 had little effect on the NIH 3T3 cell line even at the highest concentration of 100µg/ml. This study suggests that the application of EB@TiO2 effectively occluded dentine tubules and the occlusion showed a high acid resistant stability.
合成纳米蛋壳 - 二氧化钛（EB@TiO₂）生物复合材料并评估其封闭开放牙本质小管的有效性具有重要意义。采用X射线衍射（XRD）和透射电子显微镜（TEM）对EB@TiO₂进行了合成与表征。制备了16个模拟牛牙本质圆盘，并根据以下处理随机分为四组（n = 4）：第1组：不处理；第2组：蛋壳粉；第3组：EB@TiO₂；第4组：玻璃离子水门汀泡沫。然后将它们在1g粉末和40mL水的溶液中搅拌3小时。此后，将各组的每个牙本质圆盘用2wt%的柠檬酸后处理5分钟，以测试其耐酸特性。使用扫描电子显微镜（SEM）观察预处理和后处理后牙本质封闭的有效性。使用NIH 3T3试验测试合成的EB@TiO₂的细胞毒性。采用方差分析评估封闭面积比的平均值和MTS试验数据。随后进行Bonferroni校正的多重比较检验（α = 0.05）。XRD证实EB@TiO₂通过球磨成功改性。TEM显示存在球形和不规则颗粒形状的粉末。SEM结果表明EB@TiO₂可以有效地封闭开放的牙本质小管。同样，结果表明EB@TiO₂在后处理后表现出最高的耐酸稳定性。NIH 3T3试验表明，即使在最高浓度100µg/ml时，EB@TiO₂对NIH 3T3细胞系的影响也很小。本研究表明，EB@TiO₂的应用有效地封闭了牙本质小管，并且这种封闭表现出高耐酸稳定性。
