Taha Sahar T, Han Hua, Chang Sywe-Ren, Sovadinova Iva, Kuroda Kenichi, Langford Richard M, Clarkson Brian H
Department of Conservative Dentistry, The University of Jordan, Amman, Jordan.
, 1000 Shady Creek Pl, Danville, CA, 94526, USA.
Clin Oral Investig. 2015 Nov;19(8):1921-30. doi: 10.1007/s00784-015-1427-2. Epub 2015 Feb 14.
Dentin hypersensitivity (DH) is a prevalent problem. This study aimed to formulate a paste using fluorhydroxyapatite (FA) crystals dispersed in different carriers to treat DH. The ability to occlude patent dentinal tubules and to release ions was investigated.
Twenty percent FA/sodium alginate, 40% FA/poly(hydroxyethyl methacylate(HEMA)), and 40% FA/poly(DMA-co-MEA) were applied to etched dentin samples and examined with scanning electron microscopy (SEM) to determine the degree of tubule occlusion. Fluoride electrode was used to measure F release and spectroscopy to evaluate Ca and PO4 release. The cytotoxicity of the synthesized poly(DMA-co-MEA) gel was tested. Kruskall-Wallis test was used to test the differences in ion release between the groups.
FA/poly(DMA-co-MEA) paste obstructed up to 80% of the dentinal tubules, while the coverage was up to 70% for FA/poly(HEMA) and less than 50% for FA/sodium alginate. Fluoride and Ca release was the highest for FA/P(HEMA), 7.2 ± 0.7 and 139.8 ± 32.5 ppm, respectively. The highest concentration of PO4 was 46.2 ± 16.4 ppm for FA/Sodium alginate. No statistical significance was found.
FA/Poly(DMA-co-MEA) and FA/poly(HEMA) pastes may offer immediate short-term relief of DH because of their ability to occlude the tubules and adhere to wet dentin surfaces. The release of the F, Ca, and PO4 ions may offer long-term relief by forming a mineral barrier both within the dentinal tubules and on the dentin surface.
The tested materials may offer a long-term treatment for DH.
牙本质过敏症（DH）是一个普遍存在的问题。本研究旨在制备一种糊剂，该糊剂使用分散在不同载体中的氟羟基磷灰石（FA）晶体来治疗DH。研究了其封闭开放牙本质小管和释放离子的能力。
将20% FA/海藻酸钠、40% FA/聚（甲基丙烯酸羟乙酯（HEMA））和40% FA/聚（DMA-co-MEA）应用于酸蚀后的牙本质样本，并用扫描电子显微镜（SEM）检查以确定小管封闭程度。使用氟电极测量氟释放量，并用光谱法评估钙和磷酸根的释放量。测试合成的聚（DMA-co-MEA）凝胶的细胞毒性。采用Kruskal-Wallis检验来测试各组之间离子释放的差异。
FA/聚（DMA-co-MEA）糊剂可封闭高达80%的牙本质小管，而FA/聚（HEMA）的覆盖率高达70%，FA/海藻酸钠的覆盖率小于50%。FA/P（HEMA）的氟和钙释放量最高，分别为7.2±0.7和139.8±32.5 ppm。FA/海藻酸钠的磷酸根最高浓度为46.2±16.4 ppm。未发现统计学意义。
FA/聚（DMA-co-MEA）和FA/聚（HEMA）糊剂可能因其封闭小管和粘附于湿润牙本质表面的能力而提供DH的即时短期缓解。氟、钙和磷酸根离子的释放可能通过在牙本质小管内和牙本质表面形成矿物屏障而提供长期缓解。
所测试的材料可能为DH提供长期治疗。
