Xiamen Key Laboratory of Stomatological Disease Diagnosis and Treatment, Stomatological Hospital of Xiamen Medical College, No. 1309, Lvling Road, Huli District, Xiamen, 361008, Fujian, China.
Engineering Research Center of Fujian University for Stomatological Biomaterials, Xiamen, 361008, Fujian, China.
BMC Oral Health. 2022 Feb 23;22(1):43. doi: 10.1186/s12903-021-01989-z.
To synthesize mesoporous titanium dioxide composite hydroxyapatite (TiO-HAP) and to evaluate its effectiveness in sealing of occluding dentine tubules.
TiO-HAP was synthesized by chemical precipitation method and characterized using infrared absorption spectrometer, X-ray diffraction, scanning electron microscope, and specific surface area detector. Forty completely extracted molars were prepared and randomly assigned into Control group, Gluma group, HAP group and TiO-HAP group according to different treatments. The characteristics of HAP and TiO-HAP and the sealing effectiveness of dentine tubules in these four groups, including infrared spectrum, surface contact angle, pore size distribution, and re-mineralized enamel surface profiles, were analyzed by suitable characterized techniques. The cytotoxicity of the synthesized TiO-HAP was tested and compared using 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) colorimetry.
Our results showed TiO-HAP group had significantly lower contact angle, higher specific surface area, and wider range of pore size distribution than other groups. The majority of dentinal tubules in the TiO-HAP group were blocked by white matter in a uniformed manner, and the crystals arranged in order grew along the axial direction. In addition, no significant difference in optical density (OD) value was found between control group and TiO-HAP group (P > 0.05), and cell growth was good in TiO-HAP group, indicating no cytotoxicity of TiO-HAP.
The MTT assay identified that TiO-HAP had little effect on the L929 cell line. We showed TiO-HAP might be used as a remineralization agent in enamel caries-like lesions.
合成介孔二氧化钛复合羟基磷灰石（TiO-HAP）并评价其封闭牙本质小管的效果。
采用化学沉淀法合成 TiO-HAP，采用红外吸收光谱仪、X 射线衍射仪、扫描电子显微镜和比表面积测定仪对其进行表征。将 40 颗完全拔出的磨牙按不同处理随机分为对照组、Gluma 组、HAP 组和 TiO-HAP 组。采用合适的特征技术分析 HAP 和 TiO-HAP 的特征以及 4 组牙本质小管的封闭效果，包括红外光谱、表面接触角、孔径分布和再矿化釉质表面形貌。采用 3-（4,5）-二甲基噻唑（-z-y1）-3,5-二苯基四氮唑溴盐（MTT）比色法检测合成的 TiO-HAP 的细胞毒性并进行比较。
结果表明，TiO-HAP 组的接触角显著降低，比表面积显著增大，孔径分布范围更广。TiO-HAP 组的大部分牙本质小管被均匀的白色物质阻塞，晶体沿轴向有序排列生长。此外，对照组和 TiO-HAP 组的光密度（OD）值无显著性差异（P＞0.05），TiO-HAP 组细胞生长良好，表明 TiO-HAP 无细胞毒性。
MTT 检测表明 TiO-HAP 对 L929 细胞系影响较小。我们表明 TiO-HAP 可作为牙釉质龋样病变的再矿化剂。
