Guo Chuang, Wu Jiacheng, Zeng Yiming, Li Hong
Department of Materials Science and Engneering, College of Chemistry and Materials Science, Jinan University, Guangzhou, Guangdong 511436, China.
Ministry of Education, Engineering Centre of Artificial Organs and Materials, Guangzhou, Guangdong 510632, China.
Regen Biomater. 2023 Aug 11;10:rbad067. doi: 10.1093/rb/rbad067. eCollection 2023.
Reconstruction of bone defects remains a clinical challenge, and 3D bioprinting is a fabrication technology to treat it via tissue engineering. Collagen is currently the most popular cell scaffold for tissue engineering; however, a shortage of printability and low mechanical strength limited its application via 3D bioprinting. In the study, aiding with a gelatin support bath, a collagen-based scaffold was fabricated via 3D printing, where hydroxyapatite (HAP) and bone marrow mesenchymal stem cells (BMSCs) were added to mimic the composition of bone. The results showed that the blend of HAP and collagen showed suitable rheological performance for 3D extrusion printing and enhanced the composite scaffold's strength. The gelatin support bath could effectively support the HAP/collagen scaffold's dimension with designed patterns at room temperature. BMSCs in/on the scaffold kept living and proliferating, and there was a high alkaline phosphate expression. The printed collagen-based scaffold with biocompatibility, mechanical properties and bioactivity provides a new way for bone tissue engineering via 3D bioprinting.
骨缺损的修复仍然是一项临床挑战，而3D生物打印是一种通过组织工程来治疗骨缺损的制造技术。胶原蛋白是目前组织工程中最常用的细胞支架；然而，可打印性不足和机械强度低限制了其在3D生物打印中的应用。在本研究中，借助明胶支撑浴，通过3D打印制备了一种基于胶原蛋白的支架，其中添加了羟基磷灰石（HAP）和骨髓间充质干细胞（BMSCs）以模拟骨的组成。结果表明，HAP与胶原蛋白的混合物表现出适合3D挤出打印的流变性能，并增强了复合支架的强度。明胶支撑浴能够在室温下有效地支撑具有设计图案的HAP/胶原蛋白支架的尺寸。支架内/上的BMSCs保持存活和增殖，并且有高碱性磷酸酶表达。这种具有生物相容性、机械性能和生物活性的打印胶原蛋白基支架为通过3D生物打印进行骨组织工程提供了一种新方法。
