Zhou Xiaosong, Guo Min, Wang Zongliang, Wang Yu, Zhang Peibiao
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.
Regen Biomater. 2024 Aug 26;11:rbae099. doi: 10.1093/rb/rbae099. eCollection 2024.
Bioactive microspheres coated with acellular extracellular matrix (aECM) have received extensive attention in bone tissue engineering. In this work, biomimetic microspheres with different aECM ratios, uniform size and controllable size were prepared easily by blending natural porcine dermal aECM and poly (lactic-co-glycolic acid) (PLGA) using electrohydrodynamic spraying and solidification actuated by solvent extraction method. In this work, the appropriate polymer concentration and preparation voltage were investigated, and the surface morphology of the microspheres was observed by scanning electron microscope. Sirius red was used to visualize aECM exposure on the surface of the microspheres. The and experiments were carried out to evaluate the bioactivity and osteogenic properties of the microspheres. The results showed that the morphology and size of PLGA microspheres had little influence on the aECM blending. experiments showed that the higher the content of aECM, the better the cell adhesion performance. , rat calvarial defect models were observed and characterized at 4 and 8 weeks postoperatively, and the values of BV/TV of 50aECM/PLGA were 47.57 ± 1.14% and 72.92 ± 2.19%, respectively. The results showed that the skull healing effect was better in aECM-containing microspheres. In conclusion, aECM/PLGA composite microspheres can increase cell adhesion performance through the addition of aECM. Moreover, experiments have proved that aECM/PLGA microspheres are beneficial to bone repair, which means the aECM/PLGA microspheres are a promising bone tissue engineering material.
涂覆有脱细胞细胞外基质（aECM）的生物活性微球在骨组织工程中受到了广泛关注。在本研究中，通过采用静电液滴喷射法并结合溶剂萃取法引发的凝固过程，将天然猪真皮aECM与聚乳酸 - 乙醇酸共聚物（PLGA）混合，轻松制备出具有不同aECM比例、尺寸均匀且大小可控的仿生微球。在本研究中，研究了合适的聚合物浓度和制备电压，并通过扫描电子显微镜观察微球的表面形态。使用天狼星红来可视化微球表面的aECM暴露情况。进行了 和 实验以评估微球的生物活性和成骨特性。结果表明，PLGA微球的形态和尺寸对aECM混合影响不大。 实验表明，aECM含量越高，细胞黏附性能越好。 ，在术后4周和8周观察并表征大鼠颅骨缺损模型，50aECM/PLGA的骨体积分数（BV/TV）值分别为47.57±1.14%和72.92±2.19%。结果表明，含aECM的微球对颅骨愈合效果更好。总之，aECM/PLGA复合微球可通过添加aECM提高细胞黏附性能。此外， 实验证明aECM/PLGA微球有利于骨修复，这意味着aECM/PLGA微球是一种有前景的骨组织工程材料。
