Gerin Donato, Moncini Lorenzo, Faretra Francesco, Pollastro Stefania, Chimienti Nicola, Simone Gabriele, De Miccolis Angelini Rita Milvia
Department of Soil, Plant and Food Sciences, University of Bari and Selge Network, 70126 Bari, Italy.
Centro Ricerche Strumenti Biotecnici nel settore Agricolo-forestale (CRISBA), c/o ISIS "Leopoldo II di Lorena" Cittadella dello Studente, 58100 Grosseto, Italy.
Plant Dis. 2024 Feb;108(2):451-460. doi: 10.1094/PDIS-05-23-0925-RE. Epub 2024 Feb 19.
, the causal agent of pomegranate crown rot, twig blight, and fruit decay, is an emerging worldwide pathogen with a heavy impact on pomegranate cultivation. In this study, we report the rapid spread of the fungus in Italian pomegranate orchards associated with crown rot symptoms and provide new results on fungal development, baseline sensitivity to different fungicides, and intraspecific variability by analyzing 11 isolates, representative of populations of the pathogen from comparable pomegranate orchards in different regions of Italy. In vitro assays showed that 25 to 30°C was the optimal range for both colony growth and conidial germination, corroborating the results previously obtained for Californian and Greek isolates. According to the baseline sensitivity assay on the response of colony growth and conidial germination to 10 fungicides, fludioxonil, thiophanate-methyl, tebuconazole, and cyprodinil were the most effective. Random amplified polymorphic DNA (RAPD) analysis, carried out using fourteen 10-mer primers, showed very low intraspecific variability (similarity coefficient >0.95), probably as a result of the recent spread of the pathogen in Italy and the uncommon occurrence of the sexual process as a source of genetic variability. In summary, this study provides new knowledge on that will be helpful for improving pomegranate crown rot management.
石榴冠腐病、枝枯病和果实腐烂病的病原菌，是一种在全球范围内新兴的病原菌，对石榴种植造成严重影响。在本研究中，我们报告了该真菌在意大利石榴果园中与冠腐病症状相关的快速传播情况，并通过分析来自意大利不同地区可比石榴果园的病原菌种群的11个分离株，提供了关于真菌发育、对不同杀菌剂的基线敏感性以及种内变异性的新结果。体外试验表明，25至30°C是菌落生长和分生孢子萌发的最佳温度范围，这与之前对加利福尼亚和希腊分离株获得的结果一致。根据对菌落生长和分生孢子萌发对10种杀菌剂反应的基线敏感性试验，咯菌腈、甲基硫菌灵、戊唑醇和嘧菌环胺最为有效。使用14个10聚体引物进行的随机扩增多态性DNA（RAPD）分析显示种内变异性非常低（相似系数>0.95），这可能是由于该病原菌最近在意大利传播以及作为遗传变异来源的有性过程不常见所致。总之，本研究提供了关于[病原菌名称未给出]的新知识，这将有助于改善石榴冠腐病的管理。
