School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
School of International Education, Henan University of Technology, Zhengzhou 450001, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 1):126636. doi: 10.1016/j.ijbiomac.2023.126636. Epub 2023 Aug 30.
Abuse of antibiotics has led to excessive amounts of antibiotic residues in food and environment, thus enhancing pathogenic bacterium resistance and threatening human health. Therefore, searching and developing safe and green antibiotic alternatives are necessary. In this study, an Artemisia argyi leaf polysaccharide (AALP) fraction was extracted and analyzed. Chemical composition analysis showed that the carbohydrate, uronic acid, protein, and polyphenol content in AALP were 68.3 % ± 4.13 %, 9.4 % ± 0.86 %, 1.79 % ± 0.27 %, and 0.16 % ± 0.035 %, respectively. Chromatographic results suggested that AALP contained rhamnose, arabinose, glucosamine, galactose, glucose, xylose, mannose, galacturonic acid, and glucuronic acid in a molar ratio of 9.26, 1.35, 1.18, 3.04, 48.51, 2.33, 31.26, 3.93, and 9.08; the weight average molecular weight, number average molecular weight, and polydispersity of AALP were 5.41 kDa, 4.63 kDa, and 1.168, respectively. Fourier transform infrared spectroscopy indicated that AALP constituted the polysaccharide-specific groups of CH, CO, and OH. Meanwhile, AALP showed a dose-dependent inhibitory effect on Staphylococcus aureus in the inhibition zone assay, and the minimal inhibitory concentration was 1.25 mg/mL. Furthermore, AALP disrupted the cell wall, depolarized the inner membrane potential, and inhibited the activities of succinate dehydrogenase and malate dehydrogenase in S. aureus.
抗生素的滥用导致食物和环境中抗生素残留量过高，从而增强了病原菌的耐药性，威胁人类健康。因此，寻找和开发安全、绿色的抗生素替代品是必要的。本研究提取并分析了艾叶叶多糖（AALP）的一个组分。化学成分分析表明，AALP 的碳水化合物、糖醛酸、蛋白质和多酚含量分别为 68.3%±4.13%、9.4%±0.86%、1.79%±0.27%和 0.16%±0.035%。色谱结果表明，AALP 中含有鼠李糖、阿拉伯糖、氨基葡萄糖、半乳糖、葡萄糖、木糖、甘露糖、半乳糖醛酸和葡萄糖醛酸，摩尔比为 9.26、1.35、1.18、3.04、48.51、2.33、31.26、3.93 和 9.08；AALP 的重均分子量、数均分子量和多分散性分别为 5.41 kDa、4.63 kDa 和 1.168。傅里叶变换红外光谱表明，AALP 构成了 CH、CO 和 OH 等多糖特异性基团。同时，AALP 在抑菌圈试验中对金黄色葡萄球菌表现出剂量依赖性抑制作用，最小抑菌浓度为 1.25 mg/mL。此外，AALP 破坏了金黄色葡萄球菌的细胞壁，使内膜电位去极化，并抑制了琥珀酸脱氢酶和苹果酸脱氢酶的活性。
