Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huangzhou 438000, China.
Department of Infectious Diseases, Huangshi Hospital of Traditional Chinese Medicine, Huangshi 435000, China.
Ultrason Sonochem. 2024 Jul;107:106932. doi: 10.1016/j.ultsonch.2024.106932. Epub 2024 May 31.
Artemisia argyi leaf polysaccharide (AALPs) were prepared through ultrasound-assisted extraction (UAE), and their antifatigue activities were evaluated. Extraction was optimized using response surface methodology (RSM), which yielded the following optimal UAE conditions: ultrasonication power of 300 W, extraction temperature of 51 °C, liquid:solid ratio of 20 mL/g, and ultrasonication time of 47 mins. The above optimal conditions resulted in the maximum extraction rate of 10.49 %. Compared with hot water extraction (HWE), UAE supported higher yields and total sugar, uronic acid, and sulfate contents of AALPs. Meanwhile, AALP prepared through UAE (AALP-U) exhibited higher stability due to its smaller particle size and higher absolute value of zeta potential than AALP prepared through HWE (AALP-H). In addition, AALP-U demonstrated stronger antioxidant activity than AALP-H. In forced swimming tests on mice, AALP-U could significantly prolong swimming time with a dose-dependent effect, increase liver and muscle glycogen levels, and improve other biochemical indices, thus showing great potential for application in functional food.
艾叶多糖（AALPs）通过超声辅助提取（UAE）制备，并评估其抗疲劳活性。使用响应面法（RSM）对提取进行了优化，得出了以下最佳 UAE 条件：超声功率 300 W，提取温度 51°C，液固比 20 mL/g，超声时间 47 分钟。在上述最佳条件下，提取率最高可达 10.49%。与热水提取（HWE）相比，UAE 支持更高的产率和总糖、糖醛酸和硫酸盐含量的 AALPs。同时，由于粒径较小和绝对值较高的zeta 电位，通过 UAE 制备的 AALP（AALP-U）比通过 HWE 制备的 AALP（AALP-H）更稳定。此外，AALP-U 表现出比 AALP-H 更强的抗氧化活性。在小鼠强迫游泳试验中，AALP-U 可显著延长游泳时间，具有剂量依赖性，增加肝和肌肉糖原水平，并改善其他生化指标，因此在功能性食品中有很大的应用潜力。
