Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India.
Mol Biol Rep. 2023 Dec;50(12):9745-9753. doi: 10.1007/s11033-023-08719-4. Epub 2023 Sep 2.
Curcuminoids are the phenolic compounds found exclusively in turmeric. Their presence is known to increase immunity and resistance against certain cancers and neurological disorders in humans also, protecting the plant itself against salinity stress.
In this experiment, we studied the expression levels of MAPK1 and DCS genes, their curcuminoid biosynthesis under salinity stress conditions so that the impact of individual genes can be understood using semi- quantitative PCR.
The expressions of the genes with respect to curcuminoid biosynthesis showed fluctuations in their band intensity values due to the production of curcuminoids, which is initiated first in the leaves followed by the rhizomes. Not all the genes responsible for the curcuminoid biosynthesis show positive regulation under salt stress conditions which is observed in response to the severity of the stress imposed on the cultivars.
In our findings, both the genes MAPK1 and DCS were down-regulated for curcuminoid biosynthesis compared to their controls in both the cultivars Vallabh Sharad and Selection 1.
姜黄素类化合物是姜黄中特有的酚类化合物。它们的存在被认为可以提高人类的免疫力和抵抗力，预防某些癌症和神经紊乱，同时还可以保护植物自身免受盐胁迫的影响。
在这项实验中，我们研究了 MAPK1 和 DCS 基因的表达水平，以及它们在盐胁迫条件下的姜黄素生物合成情况，以便通过半定量 PCR 了解单个基因的影响。
与姜黄素生物合成有关的基因的表达水平由于姜黄素的产生而出现波动，其在叶片中首先产生，然后在根茎中产生。并非所有负责姜黄素生物合成的基因在盐胁迫条件下都表现出正调控，这是对施加给品种的胁迫严重程度的响应。
在我们的研究中，与对照相比，Vallabh Sharad 和 Selection 1 两个品种中，MAPK1 和 DCS 基因的表达水平均下调，从而影响了姜黄素的生物合成。
