Department of Clinical Pathology-Hematology and Ain Shams Medical Research Institute (MASRI), Faculty of Medicine, Ain Shams University, Cairo, Egypt; Department of Genetics and Molecular Biology, Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo, Egypt; Department of Medical Research, Armed Forces College of Medicine (AFCM), Cairo, Egypt.
Department of Medical Biochemistry, Faculty of Medicine, Zagazig University, Zagazig, Egypt; Department of Science, Faculty of Preparatory Year of Health Sciences, Princess Nourah Bint Abdulrahman University (PNU), Riyadh, Saudi Arabia.
Diagn Microbiol Infect Dis. 2023 Nov;107(3):116053. doi: 10.1016/j.diagmicrobio.2023.116053. Epub 2023 Aug 9.
The study aimed to assess the diagnostic and prognostic value of 3 specific microRNAs (miRNAs) in early-onset neonatal sepsis (NS). We examined miR-1, miR-124, and miR-34a in 70 NS patients upon admission and compared them to 70 healthy controls by RT-PCR. The main finding of the study was the difference in miRNA expression levels between NS patients and controls. Higher expression levels of miR-1 and miR-124 were significantly associated with NS, while miR-34a expression was reduced. Among the studied miRNAs, miR-34a exhibited the highest specificity (97%) as a confirmatory test for NS. In the multivariate model, miR-1 and miR-124 were found to be significant predictors of disease progression or mortality. Overall, the study suggests that miR-1, miR-124, and miR-34a could serve as potential biomarkers for diagnosing and predicting outcomes in early-onset NS.
本研究旨在评估 3 种特定 microRNAs（miRNAs）在早发型新生儿败血症（NS）中的诊断和预后价值。我们通过 RT-PCR 检测了 70 例 NS 患者入院时的 miR-1、miR-124 和 miR-34a，并与 70 例健康对照进行了比较。研究的主要发现是 NS 患者和对照组之间 miRNA 表达水平的差异。miR-1 和 miR-124 的高表达水平与 NS 显著相关，而 miR-34a 的表达水平降低。在研究的 miRNA 中，miR-34a 作为 NS 的确认试验具有最高的特异性（97%）。在多变量模型中，miR-1 和 miR-124 被发现是疾病进展或死亡的显著预测因子。总体而言，该研究表明 miR-1、miR-124 和 miR-34a 可作为早期 NS 诊断和预测结局的潜在生物标志物。
