埃及类风湿关节炎患者中一些炎症小体的作用。
Role of some inflammasomes in rheumatoid arthritis patients in Egypt.
机构信息
Microbiology and Immunology Department, Faculty of Pharmacy, Minia University, Minya, Egypt.
Microbiology and Immunology Department, Faculty of Pharmacy, Sohag University, Sohag, Egypt.
出版信息
Mol Biol Rep. 2023 Nov;50(11):8809-8815. doi: 10.1007/s11033-023-08738-1. Epub 2023 Sep 2.
AIM
This study aims to demonstrate the role of some inflammasomes genes: NLRC4 (the NLR family, CARD domain-containing protein 4), NLRP1 (NLR family, pyrin domain-containing 1), ASC (Apoptosis-associated speck-like protein containing a CARD), and CASPASE-1 in the pathogenesis of Rheumatoid arthritis (RA) in Egyptian population.
MAIN METHODS
The expression level of NLRC4, NLRP1, ASC, and CASPASE-1 within PBMCs isolated from all RA subjects by quantitative real-time PCR. GAPDH gene was used as a reference gene. Measurement of serum level of IL-1β and IL-18 was performed using ELISA.
KEY FINDINGS
Results showed dysregulated inflammasomes expression that may participate in the pathogenesis of the inflammatory process of the disease.
SIGNIFICANCE
Understanding the role of inflammasomes in RA pathogenesis helps in finding promising therapy for the treatment and management of this disease.
目的
本研究旨在探讨一些炎症小体基因（NLRC4[含 NOD 样受体结构域的 CARD 蛋白 4]、NLRP1[NOD 样受体家族，pyrin 结构域包含蛋白 1]、ASC[含凋亡相关斑点样蛋白的 CARD]和 CASPASE-1）在埃及人群类风湿关节炎（RA）发病机制中的作用。
主要方法
通过实时定量 PCR 检测所有 RA 患者 PBMCs 中 NLRC4、NLRP1、ASC 和 CASPASE-1 的表达水平，GAPDH 基因作为参考基因。采用 ELISA 法检测血清中 IL-1β 和 IL-18 的水平。
主要发现
结果显示炎症小体表达失调，可能参与了疾病炎症过程的发病机制。
意义
了解炎症小体在 RA 发病机制中的作用有助于寻找治疗和管理这种疾病的有前途的治疗方法。
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