Translational Center for Regenerative Therapies (TLC-RT), Fraunhofer Institute for Silicate Research (ISC), 97082, Würzburg, Germany.
University Hospital Würzburg, Department for Tissue Engineering and Regenerative Medicine, 97070, Würzburg, Germany.
Adv Healthc Mater. 2023 Dec;12(30):e2301131. doi: 10.1002/adhm.202301131. Epub 2023 Sep 13.
Bacterial infection is a crucial complication in implant restoration, in particular in permanent skin-penetrating implants. Therein, the resulting gap between transcutaneous implant and skin represents a permanent infection risk, limiting the field of application and the duration of application. To overcome this limitation, a tight physiological connection is required to achieve a biological and mechanical welding for a long-term stable closure including self-healing probabilities. This study describes a new approach, wherein the implant is connected covalently to a highly porous electrospun fleece featuring physiological dermal integration potential. The integrative potential of the scaffold is shown in vitro and confirmed in vivo, further demonstrating tissue integration by neovascularization, extracellular matrix formation, and prevention of encapsulation. To achieve a covalent connection between fleece and implant surface, self-initiated photografting and photopolymerization of hydroxyethylmethacrylate is combined with a new crosslinker (methacrylic acid coordinated titanium-oxo clusters) on proton-abstractable implant surfaces. For implant modification, the attached fleece is directed perpendicular from the implant surface into the surrounding dermal tissue. First in vitro skin implantations demonstrate the implants' dermal integration capability as well as wound closure potential on top of the fleece by epithelialization, establishing a bacteria-proof and self-healing connection of skin and transcutaneous implant.
细菌感染是植入物修复的一个关键并发症，特别是在永久性穿透皮肤的植入物中。在此，贯穿皮肤的植入物与皮肤之间的间隙代表着持续的感染风险，限制了其应用领域和应用时间。为了克服这一局限性，需要实现紧密的生理连接，从而实现长期稳定的闭合，包括自我修复的可能性，达到生物和机械焊接。本研究描述了一种新方法，即将植入物通过共价键连接到具有生理真皮整合潜力的高度多孔的静电纺纤维垫上。支架的整合潜力在体外得到了证明，并在体内得到了证实，进一步通过新生血管形成、细胞外基质形成和防止囊封来证明组织整合。为了在纤维垫和植入物表面之间实现共价连接，将自引发的光接枝和羟乙基甲基丙烯酸酯的光聚合与新的交联剂（配位钛氧簇的甲基丙烯酸）结合在可提取质子的植入物表面上。对于植入物的修饰，附着的纤维垫从植入物表面垂直定向到周围的真皮组织中。首次体外皮肤植入物证明了植入物的真皮整合能力，以及通过上皮化在纤维垫顶部的伤口闭合潜力，从而建立了皮肤和贯穿皮肤的植入物之间的防细菌和自我修复连接。
