Department of Gastrointestinal Surgery, Shaoxing People's Hospital, Shaoxing 312000, China.
Department of Radiology, Shaoxing People's Hospital, Shaoxing 312000, China.
Mediators Inflamm. 2023 Aug 24;2023:6368893. doi: 10.1155/2023/6368893. eCollection 2023.
p53 can function as an independent and unfavorable prognosis biomarker in cancer patients. We tried to identify the key factors of the p53 signaling pathway involved in gastric cancer (GC) occurrence and development based on the genotype-tissue expression (GTEx) and the Cancer Genome Atlas (TCGA) screening. We downloaded gene expression data and clinical data of GC included in the GTEx and TCGA databases, followed by differential analysis. Then, the key factors in the p53 signaling pathway were identified, followed by an analysis of the correlation between key factors and the prognosis of GC patients. Human GC cell lines were selected for cell experiments to verify the effects of key prognostic factors on the proliferation, migration, invasion, and apoptosis of GC cells. We found 4,944 significantly differentially expressed genes (DEGs), of which 2,465 were upregulated and 2,479 downregulated in GC. Then, 27 DEGs were found to be involved in the p53 signaling pathway. GADD45B and SERPINE1 genes were prognostic high-risk genes. The regression coefficients of GADD45B and SERPINE1 were positive. GADD45B was poorly expressed, while SERPINE1 was highly expressed in GC tissues, highlighting their prognostic role in GC. The cell experiments confirmed that overexpression of GADD45B or silencing of SERPINE1 could inhibit the proliferation, migration, and invasion and augment the apoptosis of GC cells. Collectively, the p53 signaling pathway-related factors GADD45B and SERPINE1 may be key genes that participate in the development of GC.
p53 可作为癌症患者独立的不良预后生物标志物。我们试图根据基因型组织表达 (GTEx) 和癌症基因组图谱 (TCGA) 筛选，确定参与胃癌 (GC) 发生和发展的 p53 信号通路的关键因素。我们下载了 GTEx 和 TCGA 数据库中包含的 GC 的基因表达数据和临床数据，随后进行了差异分析。然后，确定了 p53 信号通路中的关键因素，并分析了关键因素与 GC 患者预后的相关性。选择人类 GC 细胞系进行细胞实验，以验证关键预后因素对 GC 细胞增殖、迁移、侵袭和凋亡的影响。我们发现 4944 个显著差异表达基因 (DEGs)，其中 GC 中 2465 个上调，2479 个下调。然后，发现 27 个 DEGs 参与了 p53 信号通路。GADD45B 和 SERPINE1 基因是预后高危基因。GADD45B 和 SERPINE1 的回归系数为正。GADD45B 在 GC 组织中表达水平较低，而 SERPINE1 表达水平较高，这突出了它们在 GC 中的预后作用。细胞实验证实，过表达 GADD45B 或沉默 SERPINE1 可抑制 GC 细胞的增殖、迁移和侵袭，并促进其凋亡。总之，p53 信号通路相关因子 GADD45B 和 SERPINE1 可能是参与 GC 发展的关键基因。
