Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
PeerJ. 2023 Aug 30;11:e15961. doi: 10.7717/peerj.15961. eCollection 2023.
Histone acetylation and deacetylation affect the patterns of gene expression in cellular differentiation, playing pivotal roles in tissue development and maintenance. For example, the intrinsic histone acetyltransferase activity of transcriptional coactivator p300 is especially required for the expression of myogenic regulatory factors including Myf5 and MyoD, and consequently for skeletal myogenesis. On the other hand, histone deacetylases (HDACs) remove the acetyl group from histones, which is critical for gene repression in stem cell fate transition. Through integrative omic analyses, we found that while some HDACs were differentially expressed at the early stage of skeletal myoblast differentiation, gene expression was significantly enhanced by nuclear receptor signaling. In addition, p300 and MyoD control expression in milieu of normal and signal-enhanced myoblast differentiation. Thus, HDAC11 may be essential to differential gene expression at the onset of myoblast differentiation.
组蛋白乙酰化和去乙酰化影响细胞分化中的基因表达模式，在组织发育和维持中发挥关键作用。例如，转录共激活因子 p300 的固有组蛋白乙酰转移酶活性对于包括 Myf5 和 MyoD 在内的肌生成调节因子的表达至关重要，因此对于骨骼肌发生至关重要。另一方面，组蛋白脱乙酰酶（HDACs）从组蛋白上去除乙酰基，这对于干细胞命运转变中的基因抑制至关重要。通过综合组学分析，我们发现虽然在骨骼肌成肌细胞分化的早期阶段有一些 HDACs 差异表达，但核受体信号显著增强了基因表达。此外，p300 和 MyoD 在正常和信号增强的成肌细胞分化环境中控制基因的表达。因此，HDAC11 可能对成肌细胞分化起始时的差异基因表达至关重要。
