休眠期对马铃薯块茎某些生理生化指标的影响。

Impact of dormancy periods on some physiological and biochemical indices of potato tubers.

机构信息

School of Agriculture, Yunnan University, Kunming, Yunnan, China.

Guangxi Key Laboratory of Agro-environment and Agric-products safety, Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, Guangxi, China.

出版信息

PeerJ. 2023 Aug 28;11:e15923. doi: 10.7717/peerj.15923. eCollection 2023.

DOI:10.7717/peerj.15923

PMID:37663286

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10470445/

Abstract

BACKGROUND

Storage of potato tubers is an essential stage of the supply chain, from farm to consumer, to efficiently match supply and demand. However, the quality and yield of potatoes are influenced by physiological changes during storage.

METHODS

This study tested the physiological and biochemical indices in three potato varieties (YunSu 108, YunSu 304 and YunSu 306) during their dormancy periods.

RESULTS

Three potato varieties with different dormancy periods were used to follow changes in starch, protein and several enzymes during storage. The starch and sugar content of the long-dormant variety (YunSu 108, LDV) were stable, whereas those of the short-dormant variety (YunSu 306, SDV) were variable. Starch synthase activity in the three varieties was initially high, then decreased; the starch content of LDV was relatively stable, that of the medium-dormant variety (YunSu 304, MDV) increased with storage time and peaked at sprouting, and that of SDV was low but variable. The sucrose synthase activity of LDV was significantly higher ( < 0.05) than MDV and SDV in the middle storage period. Two spikes were observed in the invertase activity of SDV, whereas those of MDV and LDV were stable. The reducing sugar content of LDV increased significantly before sprouting, that of MDV slowly decreased and that of SDV dropped sharply. During the whole storage period, pectinase activity in LDV did not change significantly, whereas pectinase in MDV and SDV decreased. The cellulase and protein contents initially increased and then decreased in LDV, and steadily decreased in MDV and SDV.

CONCLUSION

The metabolic indices related to starch and sugar in the LDV were relatively stable during storage, whereas those of the SDV varied greatly. SDV showed increased sucrose, reducing sugars and cellulose; LDV PCA plots clustered in the positive quadrant of PC1 and the negative quadrant of PC2, with increased protein, sucrose synthase and starch; MDV had increased soluble starch synthase.

摘要

背景

从农场到消费者，土豆块茎的储存是供应链的一个重要环节，以有效地匹配供需。然而，在储存过程中，土豆的质量和产量受到生理变化的影响。

方法

本研究测试了三个马铃薯品种（云薯 108、云薯 304 和云薯 306）在休眠期间的生理生化指标。

结果

使用三种休眠期不同的马铃薯品种来跟踪贮藏过程中淀粉、蛋白质和几种酶的变化。长休眠品种（云薯 108，LDV）的淀粉和糖含量稳定，而短休眠品种（云薯 306，SDV）的淀粉和糖含量则不稳定。三种品种的淀粉合酶活性先高后低；LDV 的淀粉含量相对稳定，中休眠品种（云薯 304，MDV）的淀粉含量随贮藏时间的增加而增加，并在发芽时达到峰值，而 SDV 的淀粉含量较低且变化较大。中贮藏期 LDV 的蔗糖合酶活性显著高于 MDV 和 SDV（<0.05）。SDV 的转化酶活性出现两个高峰，而 MDV 和 LDV 的转化酶活性则较稳定。LDV 的还原糖含量在发芽前显著增加，MDV 的还原糖含量缓慢下降，SDV 的还原糖含量急剧下降。整个贮藏期，LDV 的果胶酶活性无明显变化，而 MDV 和 SDV 的果胶酶活性下降。LDV 的纤维素酶和蛋白质含量先增加后减少，而 MDV 和 SDV 的纤维素酶和蛋白质含量则稳步减少。