Ghanbari Ali, Jalili Cyrus, Abdolmaleki Amir, Zhaleh Mohsen, Zarinkhat Armin, Akhshi Nasim
Department of Anatomical Sciences, Kermanshah University of Medical Sciences, School of Medicine, Kermanshah, Iran.
Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Avicenna J Phytomed. 2023 Jul-Aug;13(4):442-453. doi: 10.22038/AJP.2023.21872.
Mercuric chloride (Merc; HgCl) is toxic to humans and animals and contributes to environmental pollution, which usually results in nerve and systemic harm to different organs. (FV) is a medicinal plant rich in antioxidants. This research aimed to assess the FV hydroalcoholic extract effects on kidney toxicity induced by Merc.
Forty-eight male rats were divided into eight groups: the control group: received saline; the Merc group: received 0.5 ml/day of 0.5 ppm aqueous Merc; FV1, 2, and 3 groups: received 50, 100, 150 mg/kg FV, respectively; and Merc + FV1, 2, and 3 groups: received Merc and FV at three doses. The administration period was 14-days. Subsequently, kidneys and sera were cumulated from each group for the analysis. Samples were analyzed via hematoxylin-eosin staining and biochemical tests.
The rats that received Merc displayed significant decrement in the kidney index, the diameter of renal corpuscles, total antioxidant capacity levels, superoxide dismutase activity (all, p<0.01), and 150 mg/kg FV mitigated these outcomes (all, p<0.05). Urea, creatinine, nitric oxide, and the level of apoptosis revealed a significant increment in the kidney of the rats that received Merc (all, p<0.01), and 150 mg/kg FV decreased these results. Furthermore, FV ameliorated histological changes induced by Merc (all, p<0.05).
The FV hydroalcoholic extract protects the kidneys against Merc-induced nephrotoxicity. Antioxidant and anti-apoptotic FV hydroalcoholic extract properties were involved in this healing effect.
氯化汞（Merc；HgCl）对人类和动物有毒，并会造成环境污染，通常会对不同器官造成神经和全身性损害。迷迭香（FV）是一种富含抗氧化剂的药用植物。本研究旨在评估FV水醇提取物对氯化汞诱导的肾毒性的影响。
48只雄性大鼠分为八组：对照组：接受生理盐水；氯化汞组：每天接受0.5 ppm氯化汞水溶液0.5 ml；FV1、2和3组：分别接受50、100、150 mg/kg FV；氯化汞 + FV1、2和3组：接受三种剂量的氯化汞和FV。给药期为14天。随后，从每组收集肾脏和血清进行分析。通过苏木精 - 伊红染色和生化测试对样品进行分析。
接受氯化汞的大鼠肾指数、肾小体直径、总抗氧化能力水平、超氧化物歧化酶活性均显著降低（均为p<0.01），150 mg/kg FV减轻了这些结果（均为p<0.05）。尿素、肌酐、一氧化氮和细胞凋亡水平在接受氯化汞大鼠的肾脏中显著升高（均为p<0.01），150 mg/kg FV降低了这些结果。此外，FV改善了氯化汞诱导的组织学变化（均为p<0.05）。
FV水醇提取物可保护肾脏免受氯化汞诱导的肾毒性。FV水醇提取物具有抗氧化和抗凋亡特性，参与了这种治疗作用。
Zotero 插件