Ren Yue, Zhaxi Yangzhong, Ciwang Renzeng, Wang Zhengwen, Liu Mengjun
Institute of Livestock Research, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China.
Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lhasa, China.
Front Microbiol. 2023 Aug 17;14:1247609. doi: 10.3389/fmicb.2023.1247609. eCollection 2023.
Tibetan sheep can utilize high fiber feeds well. However, the mechanisms of rumen microbiota and metabolites in response to different roughage in a housed environment are still unclear. We fed Tibetan sheep with three different roughage diets: 50% whole corn silage (TS), 50% wheatgrass group (TW), and 25% each of whole corn silage and wheatgrass (TM). Subsequently, meat traits, rumen contents 16S rRNA and metabolomics were studied. The results showed that feeding wheat straw to Tibetan sheep significantly increased the abundance of bacteria such as and in the rumen. These microorganisms significantly increased metabolites such as beta-alanyl-L-lysine, butanoic acid and prostaglandin E2. Eventually, production performance, such as carcass weight and intramuscular fat and meat quality characteristics, such as color and tenderness were improved by altering the rumen's amino acid, lipid and carbohydrate metabolism. This study demonstrated that including 25% wheatgrass and 25% whole corn silage in the diet improved the performance of Tibetan sheep, revealing the effect of the diet on the performance of Tibetan sheep through rumen microorganisms and metabolites.
藏羊能够很好地利用高纤维饲料。然而，在舍饲环境下，瘤胃微生物群和代谢产物对不同粗饲料的响应机制仍不清楚。我们给藏羊饲喂三种不同的粗饲料日粮：50%全株玉米青贮（TS）、50%披碱草组（TW）以及全株玉米青贮和披碱草各25%（TM）。随后，对肉品质性状、瘤胃内容物16S rRNA和代谢组学进行了研究。结果表明，给藏羊饲喂小麦秸秆显著增加了瘤胃中诸如 和 等细菌的丰度。这些微生物显著增加了β-丙氨酰-L-赖氨酸、丁酸和前列腺素E2等代谢产物。最终，通过改变瘤胃的氨基酸、脂质和碳水化合物代谢，提高了胴体重和肌内脂肪等生产性能以及肉色和嫩度等肉品质特性。本研究表明，日粮中包含25%的披碱草和25%的全株玉米青贮可提高藏羊的生产性能，揭示了日粮通过瘤胃微生物和代谢产物对藏羊生产性能的影响。
