Department of Biochemistry, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia.
Department of Biochemistry and Biotechnology, Faculty of Sciences, Annamalai University, 608002 Annamalai Nagar, India.
Front Biosci (Landmark Ed). 2023 Aug 28;28(8):180. doi: 10.31083/j.fbl2808180.
Recent studies suggest that numerous naturally occurring agents have the potential to kill cancer cells via mitochondrial dysfunction. is a herb widely used in alternative medical systems. This study aimed to investigate the cytotoxic effect of water extract (SNWE) against Michigan Cancer Foundation-7 (MCF-7) and MD Anderson-Metastatic Breast Cancer-231 (MDA-MB-231) cells.
We used an MTT reduction assay for cytotoxicity analysis. To explore the mode of action, the cellular adenosine triphosphate (ATP) levels and mitochondrial membrane potential were analyzed using a colorimetric ATP assay and Rhodamine-123 fluorescent staining, respectively, during SNWE treatment for 72 h.
The cytotoxic effect was significant in both cell lines, with IC50 values of 4.26 µg/mL and 5.30 µg/mL in MCF-7 and MDA-MB-231 cells, respectively. The 24, 48, and 72 h treatments of 100 µg/mL SNWE showed 0.85 ± 0.07, 0.38 ± 0.1, and 0.20 ± 0.1 nM ATP in MCF-7 cells and 0.94 ± 0.07, 0.84 ± 0.2 and 0.46 ± 0.2 nM in MDA-MB-231 cells, respectively. The SNWE treatment altered the mitochondrial membrane potential (ΔΨm) in a concentration-dependent manner in both the breast cancer cell lines, to 29.6 ± 4.1% in MCF-7 and 28.7 ± 4.17% in MDA-MB-231 cells, when compared with healthy mitochondria (100% ΔΨm).
The cytotoxic effects of against breast cancer cells are associated with energy metabolism. Additional studies are warranted to test the anticancer effect of using an animal model of breast cancer.
最近的研究表明，许多天然存在的物质通过线粒体功能障碍具有杀死癌细胞的潜力。 是一种在替代医疗系统中广泛使用的草药。本研究旨在研究 水提取物（SNWE）对密歇根癌症基金会-7（MCF-7）和 MD 安德森转移性乳腺癌-231（MDA-MB-231）细胞的细胞毒性作用。
我们使用 MTT 还原测定法进行细胞毒性分析。为了探索作用模式，在 SNWE 处理 72 小时期间，使用比色 ATP 测定法分析细胞内三磷酸腺苷（ATP）水平和线粒体膜电位，并用 Rhodamine-123 荧光染色。
在两种细胞系中均观察到明显的细胞毒性作用，MCF-7 和 MDA-MB-231 细胞的 IC50 值分别为 4.26 µg/mL 和 5.30 µg/mL。100 µg/mL SNWE 处理 24、48 和 72 小时后，MCF-7 细胞中的 ATP 分别为 0.85 ± 0.07、0.38 ± 0.1 和 0.20 ± 0.1 nM，MDA-MB-231 细胞中的 ATP 分别为 0.94 ± 0.07、0.84 ± 0.2 和 0.46 ± 0.2 nM。SNWE 处理以浓度依赖的方式改变两种乳腺癌细胞系中线粒体膜电位（ΔΨm），与健康线粒体（100%ΔΨm）相比，MCF-7 中的 ΔΨm 为 29.6 ± 4.1%，MDA-MB-231 中的 ΔΨm 为 28.7 ± 4.17%。
对乳腺癌细胞的细胞毒性作用与能量代谢有关。需要进一步的研究来使用乳腺癌动物模型测试 的抗癌作用。
