Khan Haseeb A, Prasad N Rajendra, Alrokayan Salman H, Alghamdi Amani A
Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, India.
Cell Mol Biol (Noisy-le-grand). 2023 Oct 31;69(10):136-142. doi: 10.14715/cmb/2023.69.10.19.
Breast cancer is the most progressive cancer among women worldwide. The currently available chemotherapeutic agents induce severe unacceptable adverse effects in breast cancer patients. In this context, natural medicinal herbs are gaining importance to find non-toxic effective anticancer drugs. Solanum nigrum is one of the major traditional medicinal plants widely used in Ayurveda for the treatment of various diseases. This study investigated the anticancer effect of Solanum nigrum water extract (SNWE) against MCF-7 and triple-negative MDA-MB-231 breast cancer cell lines. SNWE significantly induced oxidative stress-mediated apoptotic cell death in a concentration-dependent manner. Real-time PCR results illustrated the upregulation of proapoptotic genes and downregulation of antiapoptotic genes after SNWE treatment in MCF-7 and MDA-MB-231 cell lines. Immunofluorescence analysis showed increased expressions of apoptotic markers like p53, Caspase3 and BAX by SNWE treatment. In conclusion, the findings of this study indicate the antiproliferative effect and apoptosis-inducing property of SNWE in both cell lines. Further studies are warranted on testing the anticancer activity of S. nigrum L. using animal models of cancer.
乳腺癌是全球女性中最具侵袭性的癌症。目前可用的化疗药物会在乳腺癌患者中引发严重且难以接受的不良反应。在此背景下，天然药草在寻找无毒有效的抗癌药物方面正变得愈发重要。龙葵是阿育吠陀医学中广泛用于治疗各种疾病的主要传统药用植物之一。本研究调查了龙葵水提取物（SNWE）对MCF-7和三阴性MDA-MB-231乳腺癌细胞系的抗癌作用。SNWE以浓度依赖的方式显著诱导氧化应激介导的凋亡性细胞死亡。实时PCR结果表明，在MCF-7和MDA-MB-231细胞系中，SNWE处理后促凋亡基因上调，抗凋亡基因下调。免疫荧光分析显示，SNWE处理后凋亡标志物如p53、Caspase3和BAX的表达增加。总之，本研究结果表明SNWE在两种细胞系中均具有抗增殖作用和诱导凋亡的特性。有必要进一步开展研究，利用癌症动物模型测试龙葵的抗癌活性。
