Molecular Cardiology Unit, Department of Biochemistry, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, 625021, India.
Center for Molecular and Translational Medicine, Georgia State University, Atlanta, GA, USA.
Mol Biol Rep. 2023 Nov;50(11):8855-8866. doi: 10.1007/s11033-023-08780-z. Epub 2023 Sep 4.
Diabetic cardiomyopathy (DCM) is a form of cardiac dysfunction caused by diabetes, increasing heart failure and death. Studies shown that hyperglycemia-induced oxidative stress significantly affects heart structure and functional changes during diabetic cardiomyopathy. Fucoidans are sulfated polysaccharide derived from naturally available seaweeds and reported for various biological functions such as antioxidant, anti-diabetic, and anti-inflammatory. However, the therapeutic potential of Indian seaweeds against DCM remains largely unexplored. Therefore, the current study aimed to work on the cardioprotective effect of extracted fucoidan from Sargassum wightii (SwF) in alloxan-induced DCM.
Diabetes (DM) was induced with alloxan monohydrate (150 mg/kg) dissolved in Nacl (0.9%) overnight-fasted rats. Group III, IV rats were DM induced, followed by treated with SwF (150 mg/kg) and (300 mg/kg). Group V and VI were non-diabetic rats and received SwF (150 mg/kg) and (300 mg/kg). SwF reduced classical progressive DM complications such as hyperglycemia, polydipsia, polyphagia, and polyurea in alloxan-induced diabetic rats. Biochemical analysis showed that SwF decreased blood glucose, cardiac markers enzymes, and lipid peroxidation levels compared to diabetic rats. SwF administration significantly increased Nrf2, HO-1, SOD, Catalase, and NQO1 gene expression. In addition, SwF-treated rats showed reduced heart tissue damage with increased Nrf2 and HO-1 protein expression.
The current research concludes that targeting oxidative stress with SwF provided an effective role in the prevention of DCM. Thus, fucoidan could be used to develop functional food ingredients for DCM.
糖尿病心肌病（DCM）是一种由糖尿病引起的心脏功能障碍，增加心力衰竭和死亡的风险。研究表明，高血糖诱导的氧化应激对糖尿病心肌病期间的心脏结构和功能变化有显著影响。褐藻胶是一种从天然海藻中提取的硫酸多糖，具有抗氧化、抗糖尿病和抗炎等多种生物学功能。然而，印度海藻对 DCM 的治疗潜力在很大程度上仍未得到探索。因此，本研究旨在研究 Sargassum wightii（SwF）中提取的褐藻胶对链脲佐菌素诱导的 DCM 的心脏保护作用。
糖尿病（DM）通过在禁食过夜的大鼠中用 150mg/kg 一水合硫酸链脲佐菌素（溶于 0.9% NaCl）诱导。第 III 组和第 IV 组大鼠诱导 DM，然后用 SwF（150mg/kg）和（300mg/kg）治疗。第 V 组和第 VI 组为非糖尿病大鼠，给予 SwF（150mg/kg）和（300mg/kg）。SwF 降低了链脲佐菌素诱导的糖尿病大鼠的经典进行性 DM 并发症，如高血糖、多饮、多食和多尿。生化分析表明，与糖尿病大鼠相比，SwF 降低了血糖、心脏标志物酶和脂质过氧化水平。SwF 给药显著增加了 Nrf2、HO-1、SOD、Catalase 和 NQO1 基因的表达。此外，SwF 处理的大鼠表现出心脏组织损伤减少，同时 Nrf2 和 HO-1 蛋白表达增加。
本研究得出结论，用 SwF 靶向氧化应激在预防 DCM 方面发挥了有效作用。因此，褐藻胶可用于开发 DCM 的功能性食品成分。
