Tissue Microenvironment (TME) Lab. Aragón Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain.
Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Donostia-San Sebastián, Spain.
Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):466-475. doi: 10.1080/21691401.2023.2253534.
Tissue barrier permeability plays a crucial role in determining the selective transport of substances across epithelial tissues, including drugs, cosmetic substances, and chemicals. The ability of these substances to cross through tissue barriers affects their absorption into the bloodstream and ultimately their effectiveness. Therefore, the determination of their permeability on these type of tissue barriers represents a useful tool for pharmaceutical and cosmetic industries as well as for toxicological studies.In this regard, microfluidic devices and organ-on-chip technologies are becoming more important to generate reliable data. We have designed and performed an alternative new stratified epithelia-on-chip model that allows to correlate the Stokes radius and the diffusion of molecules and/or nanoformulations through the generated barrier and establish a system suitable for the analysis of diffusion through stratified epithelium. Thus, extrapolating from experimental data we can predict the Stokes radius for unknown fluorescent labelled particles within a molecular size range, such as gold nanoparticles.
组织屏障通透性在决定物质跨上皮组织的选择性运输中起着至关重要的作用，这些物质包括药物、化妆品物质和化学物质。这些物质穿过组织屏障的能力影响它们被吸收到血液中的程度，最终影响它们的效果。因此，确定它们在这些类型的组织屏障上的通透性对于制药和化妆品行业以及毒理学研究来说是一种有用的工具。
在这方面，微流控装置和类器官芯片技术对于生成可靠的数据变得越来越重要。我们设计并进行了一种替代的新型分层上皮细胞芯片模型，该模型允许我们将 Stokes 半径和分子以及纳米制剂的扩散与生成的屏障相关联，并建立一个适合分析通过分层上皮扩散的系统。因此，我们可以从实验数据中推断出未知荧光标记粒子在分子尺寸范围内的 Stokes 半径，例如金纳米粒子。
