Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention , Fort Collins, Colorado, USA.
Microbiol Spectr. 2023 Oct 17;11(5):e0196623. doi: 10.1128/spectrum.01966-23. Epub 2023 Sep 5.
Orthobunyaviruses in the California serogroup cause severe neurological disease in children and adults. While these viruses are known to circulate widely in North America, their occurrence is rare. Serological testing for CSGVs is hindered by the limited availability and volumes of human-positive specimens needed as controls in serologic assays. Here, we described the development of a murine monoclonal antibody cross-reactive to CSGVs engineered to contain the variable regions of the murine antibody on the backbone of human IgM. The chimeric IgM produced from the stably expressing HEK293 cell line was evaluated for use as a surrogate human-positive control in a serologic diagnostic test.
加利福尼亚血清群正粘病毒可导致儿童和成人的严重神经系统疾病。虽然这些病毒在北美广泛传播，但它们的发生较为罕见。由于在血清学检测中需要有限的、人类阳性标本的数量作为对照，因此针对 CSGV 的血清学检测受到限制。在这里，我们描述了一种对 CSGV 具有交叉反应性的鼠单克隆抗体的开发，该抗体工程化后在人 IgM 的骨架上包含了鼠抗体的可变区。从稳定表达的 HEK293 细胞系中产生的嵌合 IgM，在血清学诊断检测中被评估用作替代的人类阳性对照。
