CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.
CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, China.
Nano Lett. 2023 Sep 27;23(18):8568-8575. doi: 10.1021/acs.nanolett.3c02348. Epub 2023 Sep 5.
The widespread dissemination of ultraflexible neural probes depends on the development of advanced materials and implementation strategies that can allow reliable implantation of ultraflexible neural probes into targeted brain regions, especially deep and difficult-to-access brain regions. Here, we report ultraflexible and multidirectional probes that are encapsulated in a biocompatible polymer alloy with controllable dissolution kinetics. Our probes can be reliably implanted into targeted brain regions over large spatial scales, including deep hindbrain regions that are anatomically difficult-to-access in vivo. Chronically implanted probes can enable long-term, multidirectional recordings from several hundreds of neurons across distributed brain regions. In particular, our results show that 87.0% of chronically recorded neurons in the hindbrain are interneurons, whereas only 41.9% of chronically recorded neurons in the cortex are interneurons. These results demonstrate that our ultraflexible neural probes are a promising tool for large-scale, long-term neural circuit dissection in the brain.
超柔韧神经探针的广泛传播取决于先进材料和实施策略的发展，这些策略可以允许将超柔韧神经探针可靠地植入到目标大脑区域，特别是深部和难以到达的大脑区域。在这里，我们报告了一种封装在具有可控溶解动力学的生物相容性聚合物合金中的超柔韧和多向探针。我们的探针可以可靠地植入到包括深部后脑区域在内的大空间尺度的目标大脑区域，而这些区域在体内解剖上是难以到达的。慢性植入的探针可以实现来自分布在大脑区域的数百个神经元的长期、多向记录。特别是，我们的结果表明，慢性记录的后脑神经元中有 87.0%是中间神经元，而皮质中慢性记录的神经元只有 41.9%是中间神经元。这些结果表明，我们的超柔韧神经探针是在大脑中进行大规模、长期神经回路剖析的有前途的工具。
