New York University Neuroscience Institute, School of Medicine, New York University, New York, New York;
J Neurophysiol. 2014 Mar;111(5):1132-49. doi: 10.1152/jn.00785.2013. Epub 2013 Dec 18.
Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits free behavior of small rodents. The system integrates multishank, high-density recording silicon probes, ultraflexible interconnects, and a miniaturized microdrive. These improvements allowed for simultaneous recordings of local field potentials and unit activity from hundreds of sites without confining free movements of the animal. The advantages of large-scale recordings are illustrated by determining the electroanatomic boundaries of layers and regions in the hippocampus and neocortex and constructing a circuit diagram of functional connections among neurons in real anatomic space. These methods will allow the investigation of circuit operations and behavior-dependent interregional interactions for testing hypotheses of neural networks and brain function.
在行为动物中监测来自多个脑回路的代表性神经元分数对于理解神经元计算是必要的。在这里，我们描述了一种系统，该系统使用允许小型啮齿动物自由行为的轻量级信号多路复用前置放大器，可从小体积的神经元组织中进行高通道计数记录。该系统集成了多针、高密度记录硅探针、超灵活的互连和微型微驱动器。这些改进允许在不限制动物自由运动的情况下，同时从数百个部位记录局部场电位和单元活动。通过确定海马体和新皮层中各层和区域的电解剖边界，并在真实解剖空间中构建神经元之间的功能连接电路图，说明了大规模记录的优势。这些方法将允许研究电路操作和行为相关的区域间相互作用，以测试神经网络和大脑功能的假设。
