数字黏膜样本中结肠隐窝的数量：诊断溃疡性结肠炎的一个新的独立参数
The Number of Colon Crypts in Digital Mucosal Samples: A New Independent Parameter for Diagnosing Ulcerative Colitis.
作者信息
Rubio Carlos A, Lang-Schwarz Corinna, Matek Christian, Kamaradova Katerina, Vieth Michael
机构信息
Department of Pathology, Karolinska Institute and University Hospital, Stockholm, Sweden.
Institute of Pathology, Friedrich-Alexander University Erlangen-Nuremberg, Klinikum Bayreuth, Bayreuth, Germany.
出版信息
Cancer Diagn Progn. 2023 Sep 3;3(5):533-537. doi: 10.21873/cdp.10251. eCollection 2023 Sep-Oct.
BACKGROUND/AIM: It has been demonstrated that most routine biopsies from the colon and rectum display cross-cut crypts (CCC). The aim was to assess the number of CCC in microscopic isometric digital samples (0.500 mm) from routine colon biopsies.
PATIENTS AND METHODS
Colon biopsies from 224 patients were investigated: 99 in patients with ulcerative colitis (UC), 31 UC in remission (UCR), 28 infectious colitis (IC), 7 resolved IC (RIC), 19 diverticular sigmoiditis (DS), and 40 normal colon mucosa (NCM).
RESULTS
A total of 8,024 CCC were registered: 2,860 (35.6%) in UC, 1,319 UCR (16.4%), 849 (10.6%) in IC, 340 (4.2%) in RIC, 795 (9.9%) in DS, and 1,861 (23.2%) in NCM. The CCC frequencies in UC and IC were significantly lower (p<0.05) than those in UCR, RIC, DS, and NCM.
CONCLUSION
By the simple algorithm of counting CCC in standardized isometric microscopic digital circles measuring 0.500 mm, it was possible to differentiate between UC (long-lasting inflammation) and IC (short-lasting inflammation) on the one hand, and UCR, RIC, DS (persistent inflammation), and NCM, on the other. The counting of CCC in the algorithm by five pathologists working in three disparate European Countries, was found to be reproducible.
背景/目的：已证实，大多数来自结肠和直肠的常规活检标本显示有横切隐窝（CCC）。目的是评估常规结肠活检标本的微观等距数字样本（0.500毫米）中CCC的数量。
患者和方法
对224例患者的结肠活检标本进行了研究：99例溃疡性结肠炎（UC）患者，31例缓解期UC（UCR）患者，28例感染性结肠炎（IC）患者，7例已缓解的IC（RIC）患者，19例乙状结肠憩室炎（DS）患者，以及40例正常结肠黏膜（NCM）患者。
结果
共记录到8024个CCC：UC中有2860个（35.6%），UCR中有1319个（16.4%），IC中有849个（10.6%），RIC中有340个（4.2%），DS中有795个（9.9%），NCM中有1861个（23.2%）。UC和IC中的CCC频率显著低于UCR、RIC、DS和NCM中的频率（p<0.05）。
结论
通过在测量为0.500毫米的标准化等距微观数字圆圈中计数CCC的简单算法，可以区分一方面的UC（持续性炎症）和IC（短期炎症），以及另一方面的UCR、RIC、DS（持续性炎症）和NCM。发现在三个不同欧洲国家工作的五名病理学家对该算法中CCC的计数具有可重复性。
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