R&D, Pierre Fabre Dermo-Cosmétique et Personal Care, Toulouse, France.
Laboratoires Dermatologiques Avène, Lavaur, France.
J Eur Acad Dermatol Venereol. 2023 Oct;37 Suppl 6:6-11. doi: 10.1111/jdv.19243.
Overexposure to sunlight can have many harmful biological effects on the skin, leading to skin cancer and photoaging. As ultraviolet (UV) radiation has been identified as a cause of DNA damage and oxidative stress in the skin, the photoprotection provided by sunscreens is evaluated through their ability to filter UV light, using the sun protection factor (SPF). However, recent data have shown that high-energy visible (HEV) light can also cause biological skin damage.
To develop a new in vivo method for evaluating the protection provided by sunscreens across a broad range of wavelengths, including the HEV band, based on multispectral image analysis.
This study evaluated the absorption properties of six commercially available sunscreens (five SPF 50+ products containing organic UV filters, and one product containing the wide spectrum filter, phenylene bis-diphenyltriazine [TriAsorB™]) and of a control product containing no filter. Multispectral images were acquired from the skin on the forearms of healthy volunteers, before and after application of the test products. Images taken with LEDs emitting light at wavelengths ranging from UV to infrared were used to generate light reflectance maps for each product. The levels of absorbance of light in the UV and visible bands were then calculated.
The product containing the wide spectrum filter exhibited significantly higher absorbance over the HEV band (380-450 nm) than the control product and the other commercial sunscreens. All the sunscreens tested showed the same level of absorbance at 365 nm (UVA).
Multispectral imaging provides a simple and reliable in vivo method for assessing the real-world protection provided by sunscreens against all forms of photo-induced skin damage, including that induced by HEV radiation.
过度暴露在阳光下会对皮肤产生许多有害的生物学影响，导致皮肤癌和光老化。由于紫外线 (UV) 辐射已被确定为皮肤中 DNA 损伤和氧化应激的原因，因此防晒霜的光保护作用是通过其过滤 UV 光的能力来评估的，使用防晒因子 (SPF)。然而，最近的数据表明，高能可见光 (HEV) 也会导致皮肤的生物损伤。
开发一种新的体内方法，用于评估包括 HEV 波段在内的广谱波长范围内防晒霜提供的保护，该方法基于多光谱图像分析。
本研究评估了六种市售防晒霜（五种含有有机 UV 滤光片的 SPF50+产品，以及一种含有宽谱滤光片二苯甲酮的产品）和一种不含滤光片的对照产品的吸收特性。在健康志愿者的前臂皮肤上涂抹测试产品前后，采集多光谱图像。使用发出从 UV 到红外光的 LED 拍摄的图像，为每个产品生成光反射率图。然后计算紫外线和可见光波段的光吸收率。
含有宽谱滤光片的产品在 HEV 波段（380-450nm）的吸收率明显高于对照产品和其他市售防晒霜。所有测试的防晒霜在 365nm（UVA）处的吸收率相同。
多光谱成像为评估防晒霜在现实世界中提供的针对所有形式的光诱导皮肤损伤（包括由 HEV 辐射引起的损伤）的实际保护提供了一种简单可靠的体内方法。
