School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei 230601, Anhui, China.
Food Funct. 2023 Sep 19;14(18):8646-8660. doi: 10.1039/d3fo03093h.
is a potential probiotic that possesses beneficial health properties and fermentation traits; however, the extent of understanding of the antioxidant activities of is limited. This study investigates the antioxidant activities of a new FFZZH5L strain. The strain exhibits broad tolerance to acids, bases and salts and demonstrated good adaption to the gastrointestinal environment, with a survival rate of 45% after 24 h of treatment in artificial gastrointestinal juice. Moreover, FFZZH5L exhibits inhibitory effects on , with a self-aggregation rate of 34.8% and a co-aggregation rate of 82.2%. , the DPPH radical scavenging ability and GSH-px enzyme activity of FFZZH5L reach 64.27% and 15.95 U mL, respectively. Treatment of with FFZZH5L significantly extended the organism's lifespan. Furthermore, the activity of SOD, GSH-px and T-AOC was increased by 33.6%, 43.4% and 58.3%, respectively. Feeding with FFZZH5L decreased the MDA, lipofuscin and ROS levels by 9%-36.4%. FFZZH5L effectively protected against juglone-induced oxidative stress damage and led to a significant increase in the organism's survival under heat stress. The RT-qPCR analysis suggests that feeding with FFZZH5L upregulates the expression levels of antioxidant-related genes including glutathione -transferase 4 (), , , , , and in . Our investigation confirms the probiotic and antioxidant properties of , indicating its potential application in functional foods and the pharmaceutical industry.
是一种具有有益健康特性和发酵特性的潜在益生菌；然而，对 的抗氧化活性的理解程度有限。本研究调查了一种新的 FFZZH5L 菌株的抗氧化活性。该菌株对酸、碱和盐具有广泛的耐受性，并能很好地适应胃肠道环境，在人工胃液中处理 24 小时后存活率为 45%。此外，FFZZH5L 对 具有抑制作用，自聚集率为 34.8%，共聚率为 82.2%。FFZZH5L 的 DPPH 自由基清除能力和 GSH-px 酶活性分别达到 64.27%和 15.95 U mL。用 FFZZH5L 处理 显著延长了生物的寿命。此外，SOD、GSH-px 和 T-AOC 的活性分别增加了 33.6%、43.4%和 58.3%。用 FFZZH5L 喂养可使 MDA、脂褐质和 ROS 水平降低 9%-36.4%。FFZZH5L 能有效保护 免受胡桃醌诱导的氧化应激损伤，并显著提高机体在热应激下的存活率。RT-qPCR 分析表明，用 FFZZH5L 喂养能上调抗氧化相关基因的表达水平，包括谷胱甘肽转移酶 4 (), 、 、 、 、 和 在 中的表达。我们的研究证实了 的益生菌和抗氧化特性，表明其在功能性食品和制药工业中有潜在的应用。
