Zou Sibo, Wu Qi, Li Zhigao, Zhang Sufang, Dong Liang, Chen Yingxi, Dai Yiwei, Ji Chaofan, Liang Huipeng, Lin Xinping
SKL of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Collaborative Innovation Center of Provincial and Ministerial Co-Construction for Deep Processing, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Foods. 2024 Mar 19;13(6):924. doi: 10.3390/foods13060924.
This research endeavored to elucidate the antioxidant attributes of lactic acid bacteria, specifically their impact on anti-aging and lifespan augmentation in . The study focused on A72, identified through ARTP mutagenesis for its potent antioxidant properties. In vitro analysis affirmed its free radical neutralizing capacity. In , the strain not only extended the lifespan by 25.13% and amplified motility 2.52-fold, but also maintained reproductive capabilities. Remarkably, A72 diminished reactive oxygen species (ROS) and malondialdehyde (MDA) levels in by 34.86% and 69.52%, respectively, while concurrently enhancing its antioxidant enzyme activities. The strain also bolstered survival rates by 46.33% and 57.78% under high temperature and HO conditions, respectively. Transcriptomic scrutiny revealed that A72 could retard aging and extend lifespan by upregulating the and genes and downregulating the and genes. These findings propose A72 as a potential antioxidant and anti-aging lactic acid bacteria.
本研究致力于阐明乳酸菌的抗氧化特性，特别是它们对秀丽隐杆线虫抗老化和寿命延长的影响。该研究聚焦于通过常压室温等离子体诱变因其强大的抗氧化特性而被鉴定出的A72。体外分析证实了其自由基中和能力。在秀丽隐杆线虫中，该菌株不仅使寿命延长了25.13%，运动能力提高了2.52倍，还保持了繁殖能力。值得注意的是，A72使秀丽隐杆线虫中的活性氧（ROS）和丙二醛（MDA）水平分别降低了34.86%和69.52%，同时提高了其抗氧化酶活性。在高温和过氧化氢条件下，该菌株还分别将秀丽隐杆线虫的存活率提高了46.33%和57.78%。转录组学研究表明，A72可以通过上调hsp-16.2和ctl-1基因以及下调sir-2.1和age-1基因来延缓秀丽隐杆线虫的衰老并延长寿命。这些发现表明A72是一种潜在的抗氧化和抗老化乳酸菌。
