GVG Genetic Monitoring GmbH, Leipzig, Germany.
Institute of Laboratory Animal Science, University of Zurich, Switzerland.
Lab Anim. 2023 Aug;57(4):371-380. doi: 10.1177/00236772231175727.
Information about the diploid genotype of a gene-modified or mutant laboratory animal is essential for breeding and experimental planning. It is also required for the exchange of animals between different research groups or for communication with professional genotyping service providers. While there are detailed, standardized rules for creating an allele name of a genome modification or mutation, the notation of the diploid genotype after biopsy and genotyping has not been standardized yet. Therefore, a uniform, generally understandable nomenclature for the diploid genotype of gene-modified laboratory animals is needed. With the here-proposed nomenclature recommendations from the Committee on Genetics and Breeding of Laboratory Animals of the German Society for Laboratory Animal Science (GV-SOLAS), we provide a practical, standardized representation of the genotype of gene-modified animals. It is intended to serve as a compact guide for animal care and scientific personnel in animal research facilities and to simplify data exchange between groups and with external service providers.
有关基因修饰或突变实验动物的二倍体基因型的信息对于繁殖和实验规划至关重要。它也是不同研究小组之间动物交换或与专业基因分型服务提供商进行沟通所必需的。虽然创建基因组修饰或突变的等位基因名称有详细的标准化规则，但活检和基因分型后的二倍体基因型的表示尚未标准化。因此，需要一种用于基因修饰实验动物二倍体基因型的统一、易于理解的命名法。有鉴于此，德国实验动物科学学会（GV-SOLAS）遗传学和育种委员会提出了命名法建议，为基因修饰动物的基因型提供了一种实用的、标准化的表示方法。它旨在为动物研究设施中的动物护理和科研人员提供一个简明的指南，并简化组内和与外部服务提供商之间的数据交换。
