Mahmoud A A, Peters P A, Civil R H, Remington J S
J Immunol. 1979 May;122(5):1655-7.
Resistance to Schistosoma mansoni infection in the mouse has been induced either specifically by a primary infection with this parasite or nonspecifically by a variety of immunostimulants such as BCG. In the present study we developed an in vitro system to examine the effector mechanism of nonspecifically induced resistance. Activated macrophage monolayers obtained from BCG- or Corynebacterium parvum treated mice killed a respective mean 32 +/- 6% and 48 +/- 5% of schistosomula after 24 hr incubation. The killing of the parasites was verified by their inability to mature to adult worms upon injection into normal mice. The activated macrophage-mediated killing was related to cell:parasite ratio, and was partially lost if the macrophage monolayers were kept in cultures for 24 hr before incubation with the organism. Supernatants of macrophages cultured in the presence of schistosomula killed a mean of 51 +/- 3% of the organisms whereas those from cells cultured alone resulted in a mean killing of 25 +/- 3%. Furthermore, toxic supernatants could be generated equally well on incubation with S. mansoni schistosomula or Trichinella spiralis larvae. Our data show that activated macrophage monolayers through soluble mediators destroy a significant proportion of the multicellular parasite S. mansoni schistosomula in vitro.
小鼠对曼氏血吸虫感染的抵抗力可通过该寄生虫的初次感染特异性诱导产生，也可通过多种免疫刺激剂（如卡介苗）非特异性诱导产生。在本研究中，我们建立了一个体外系统来研究非特异性诱导抵抗力的效应机制。从经卡介苗或短小棒状杆菌处理的小鼠获得的活化巨噬细胞单层在孵育24小时后，分别平均杀死了32±6%和48±5%的血吸虫幼虫。将寄生虫注射到正常小鼠体内后无法发育为成虫，从而证实了寄生虫被杀死。活化巨噬细胞介导的杀伤作用与细胞与寄生虫的比例有关，如果巨噬细胞单层在与生物体孵育前在培养物中保存24小时，则杀伤作用会部分丧失。在存在血吸虫幼虫的情况下培养的巨噬细胞上清液平均杀死了51±3%的生物体，而单独培养细胞的上清液平均杀伤率为25±3%。此外，与曼氏血吸虫幼虫或旋毛虫幼虫孵育时，均可产生同样有效的毒性上清液。我们的数据表明，活化的巨噬细胞单层通过可溶性介质在体外破坏了相当比例的多细胞寄生虫曼氏血吸虫幼虫。
