School of Materials Science and Engineering, University of Jinan, Jinan, 250022, PR China.
School of Materials Science and Engineering, University of Jinan, Jinan, 250022, PR China.
Talanta. 2024 Jan 15;267:125141. doi: 10.1016/j.talanta.2023.125141. Epub 2023 Aug 30.
The facile detection of total antioxidant capacity (TAC) is limited by in-situ analysis, because it usually requires complex laboratory equipments. Here, a colorimetric assay for TAC detection is developed based on the peroxidase-like activity of iron-doped carbon nitride (Fe/NC) and the smartphone platform. The peroxidase-like activity of carbon nitride is greatly improved by the introduction of Fe atoms, and the active sites turn to Fe-N coordination groups in the Fe/NC. The inhibition mechanism of the chromogenic reaction for different kinds of antioxidants is also studied. The colorimetric assay is fabricated by the relationship of absorbance-color-antioxidant content and applied successfully to the TAC detection of several fruit juicesand commercial beverages. This work not only provides a promising approach for convenient in-situ TAC assay without the use of large instruments, but also expands the application of nanozymes in nutritional value assessment of foods.
总抗氧化能力（TAC）的简便检测受到原位分析的限制，因为它通常需要复杂的实验室设备。在这里，我们基于氮化碳的过氧化物酶样活性和智能手机平台，开发了一种用于 TAC 检测的比色分析方法。通过引入铁原子，氮化碳的过氧化物酶样活性得到了极大的提高，活性位点在 Fe/NC 中转变为 Fe-N 配位基团。还研究了显色反应中不同种类抗氧化剂的抑制机制。比色分析方法是通过吸光度-颜色-抗氧化剂含量之间的关系来构建的，并成功应用于几种果汁和商业饮料的 TAC 检测。这项工作不仅为无需使用大型仪器的方便的原位 TAC 分析提供了一种很有前途的方法，而且还扩展了纳米酶在食品营养价值评估中的应用。
