本土芽孢杆菌属对拉氏鱥生长、抗氧化能力、免疫力和抗病性的有益影响。
Beneficial effects of indigenous Bacillus spp. on growth, antioxidants, immunity and disease resistance of Rhynchocypris lagowskii.
作者信息
Elsadek Mahmoud M, Zhu Wenli, Wang Sibu, Wang Xin, Guo Zhixin, Lin Lili, Wang Guiqin, Wang Qiuju, Chen Yuke, Zhang Dongming
机构信息
College of Animal Science and Technology, Key Laboratory for Animal Production Quality and Safety of Ministry of Education, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, 130118, China; Department of Fish Production, Faculty of Agriculture, Al-Azhar University, Cairo, 11884, Egypt.
College of Animal Science and Technology, Key Laboratory for Animal Production Quality and Safety of Ministry of Education, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, 130118, China.
出版信息
Fish Shellfish Immunol. 2023 Oct;141:109047. doi: 10.1016/j.fsi.2023.109047. Epub 2023 Sep 4.
This study aimed to investigate the effect of Bacillus aryabhattai (LSG3-7) and Bacillus mojavensis (LSG3-8) on growth performance, antioxidant capacity, and immune response in Rhynchocypris lagowskii (Dybowski, 1869), at the trial and challenge periods. A 630 healthy fish (10.76 ± 0.05) were randomly divided into six groups: control group (D1) was fed the basal diet, D2 and D3 were supplemented with LSG 3-7 and LSG3-8 (1 × 10 CFU/g) for both of them, whereas D4 was supplemented with a mixture of both bacteria (0.5 × 10 CFU/g each), and D5 was supplemented with LSG3-7 0.75 × 10 CFU/g + LSG3-8 0.25 × 10 CFU/g, and D6 supplemented with LSG3-7 0.25 × 10 CFU/g + LSG3-8 0.75 × 10 CFU/g. After the trial, Aeromonas hydrophila was used in a challenge test for 14 days. Treatments showed significant differences (p < 0.05) in growth performance and antioxidant capacity (CAT, CuZn-SOD, GPX) in the liver and intestine compared to the control. The antioxidant-related genes CAT, CuZn-SOD, GPX, and Nrf2 in the liver and intestine showed upregulation compared with the control group. Serum IgM, LZM, C3, C4, and AKP showed a favorable superiority (p < 0.05) in treatments (D2 - D6) at the trial and challenge test compared to controls. In parallel, immune-related genes (IgM, NF-κB, TLR-1, TLR-2, and MyD88) showed an up-regulated level (p < 0.05) in treatments (D2 - D6) compared to the control. In addition, pro-inflammatory cytokines (IL-1, TNF-α) showed a downregulated level in treatments (D2 - D6). After the challenge test, the immune-related genes in the liver and muscle showed an up-regulated level in treatments compared to the controls. The survival rate showed a significant increase (p < 0.05) in the treatment groups (D2 - D6) compared to the control. Overall, individuals and the bacterial mixture of B. aryabhattai and B. mojavensis could improve the growth performance, antioxidant capacity, immune capacity, and survival rate of R. lagowskii and prevent side effects of A. hydrophila. However, B. mojavensis showed a slight improvement compared to B. aryabhattai without a significant difference between them.
本研究旨在调查阿氏芽孢杆菌（LSG3 - 7）和莫哈韦芽孢杆菌（LSG3 - 8）在试验期和攻毒期对拉氏鱥（Dybowski，1869）生长性能、抗氧化能力及免疫反应的影响。将630尾健康的鱼（体重10.76 ± 0.05）随机分为六组：对照组（D1）投喂基础饲料，D2和D3组均添加LSG 3 - 7和LSG3 - 8（1×10⁶CFU/g），D4组添加两种菌的混合物（各0.5×10⁶CFU/g），D5组添加LSG3 - 7 0.75×10⁶CFU/g + LSG3 - 8 0.25×10⁶CFU/g，D6组添加LSG3 - 7 0.25×10⁶CFU/g + LSG3 - 8 0.75×10⁶CFU/g。试验结束后，用嗜水气单胞菌进行攻毒试验，持续14天。与对照组相比，各处理组在生长性能以及肝脏和肠道的抗氧化能力（CAT、CuZn - SOD、GPX）方面表现出显著差异（p < 0.05）。肝脏和肠道中与抗氧化相关的基因CAT、CuZn - SOD、GPX和Nrf2与对照组相比呈上调状态。在试验期和攻毒试验中，处理组（D2 - D6）的血清IgM、LZM、C3、C4和AKP与对照组相比表现出明显优势（p < 0.05）。同时，与免疫相关的基因（IgM、NF - κB、TLR - 1、TLR - 2和MyD88）在处理组（D2 - D6）中与对照组相比呈上调水平（p < 0.05）。此外，促炎细胞因子（IL - 1、TNF - α）在处理组（D2 - D6）中呈下调水平。攻毒试验后，处理组肝脏和肌肉中的免疫相关基因与对照组相比呈上调水平。与对照组相比，处理组（D2 - D6）的存活率显著提高（p < 0.05）。总体而言，阿氏芽孢杆菌和莫哈韦芽孢杆菌的单一菌株及混合菌株均可提高拉氏鱥的生长性能、抗氧化能力、免疫能力和存活率，并预防嗜水气单胞菌的副作用。然而，莫哈韦芽孢杆菌与阿氏芽孢杆菌相比有轻微改善，但二者之间无显著差异。
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