Liu Yantao, Zhuang Zhenxiao, Liao Zhihong, Yao Rong, Chen Mengdie, Wei Hanlin, Zhao Wei, Niu Jin
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
Antioxidants (Basel). 2023 Nov 24;12(12):2042. doi: 10.3390/antiox12122042.
The aim of this study was to evaluate the effects of a low-fish-meal diet supplemented with coenzyme Q10 on the growth, antioxidant capacity, immunity, intestinal health and hypoxic resistance of . with an initial weight of 0.66 g were fed with the experimental diets for 56 days. Diets D1 (20% FM level) and D2-D7 (15% FM level), supplemented with 0%, 0.002%, 0.004%, 0.006%, 0.008% and 0.01% coenzyme Q10 were formulated. In terms of growth performance, the weight gain and specific growth rate in the D2 diet were significantly lower than those in the D1 diet ( < 0.05). The final body weight, weight gain and specific growth rate in the D2-D7 diets had an upward trend, and the condition factor in the D2-D7 diets was lower than those in the D1 diet ( < 0.05). There were no significant differences in the crude protein and crude lipid levels in the whole body among all diet treatments ( > 0.05). In terms of hepatopancreas antioxidant parameters, the D5 and D6 diets significantly promoted the total antioxidant capacity and total superoxide dismutase activity, and significantly decreased the malondialdehyde content ( < 0.05). The expression levels of , and in shrimp fed with the D5 and D6 diets were significantly higher than those of shrimp fed with the D2 diet ( < 0.05). In addition, the mRNA level of was increased in the D4 and D5 diets, and expression was increased in the D6 diet compared with the D1 diet ( < 0.05). The villus height of shrimp fed with diets supplemented with coenzyme Q10 was significantly increased ( < 0.05), and the intestinal thickness and submucosal thickness of shrimp fed with the D6 diet were the highest ( < 0.05). After acute hypoxia stress, lethal dose 50 time in the D3-D7 diets was significantly increased compared with the D1 and D2 diets ( < 0.05), and the highest value was found in the D4 diet ( < 0.05). After stress, the expression levels of TLR pathway-related genes (, , , and in the D4 and D6 diets were significantly increased compared with the D2 diet. In general, fed with the D6 diet achieved the best growth, antioxidant capacity, immunity, and intestinal morphology among all low FM diets and D4-D6 diets improved hypoxic resistance.
本研究的目的是评估添加辅酶Q10的低鱼粉日粮对初始体重为0.66克的[具体对象未给出]的生长、抗氧化能力、免疫力、肠道健康和耐缺氧性的影响。将[具体对象未给出]投喂实验日粮56天。配制了日粮D1（鱼粉水平20%）和D2 - D7（鱼粉水平15%），分别添加0%、0.002%、0.004%、0.006%、0.008%和0.01%的辅酶Q10。在生长性能方面，D2日粮中的增重和特定生长率显著低于D1日粮（P < 0.05）。D2 - D7日粮中的终末体重、增重和特定生长率呈上升趋势，且D2 - D7日粮中的肥满度低于D1日粮（P < 0.05）。所有日粮处理组鱼体的粗蛋白和粗脂肪水平无显著差异（P > 0.05）。在肝胰腺抗氧化参数方面，D5和D6日粮显著提高了总抗氧化能力和总超氧化物歧化酶活性，并显著降低了丙二醛含量（P < 0.05）。投喂D5和D6日粮的虾中[具体基因未给出]、[具体基因未给出]和[具体基因未给出]的表达水平显著高于投喂D2日粮的虾（P < 0.05）。此外，与D1日粮相比，D4和D5日粮中[具体基因未给出]的mRNA水平升高，D6日粮中[具体基因未给出]的表达升高（P < 0.05）。投喂添加辅酶Q10日粮的虾的绒毛高度显著增加（P < 0.05），投喂D6日粮的虾的肠厚度和黏膜下层厚度最高（P < 0.05）。急性缺氧应激后，D3 - D7日粮的半数致死时间与D1和D2日粮相比显著延长（P < 0.05），且D4日粮中的值最高（P < 0.05）。应激后，与D2日粮相比，D4和D6日粮中TLR途径相关基因（[具体基因未给出]、[具体基因未给出]、[具体基因未给出]、[具体基因未给出]和[具体基因未给出]）的表达水平显著升高。总体而言，在所有低鱼粉日粮中，投喂D6日粮的[具体对象未给出]生长、抗氧化能力、免疫力和肠道形态最佳，且D4 - D6日粮提高了耐缺氧性。
