海产品中副溶血性弧菌计数方法的评估
Evaluation of methods for enumeration of Vibrio parahaemolyticus from seafood.
作者信息
Karunasagar I, Venugopal M N, Karunasagar I, Segar K
出版信息
Appl Environ Microbiol. 1986 Sep;52(3):583-5. doi: 10.1128/aem.52.3.583-585.1986.
Abstract
The efficiency of several enrichment broths in recovering Vibrio parahaemolyticus inoculated into fish homogenates was studied. Recovery by the most probable number technique was very low in all the broths, while direct plating on thiosulfate citrate bile salt sucrose agar yielded better recovery. A decrease in the enrichment time to 8 from 18 h did not improve recovery. At concentrations exceeding 2.5 micrograms/ml, polymyxin was inhibitory to V. parahaemolyticus.
摘要
研究了几种增菌肉汤在从接种到鱼匀浆中的副溶血性弧菌中回收细菌的效率。通过最可能数技术在所有肉汤中的回收率都非常低，而直接接种到硫代硫酸盐柠檬酸盐胆盐蔗糖琼脂上的回收率更高。将增菌时间从18小时减少到8小时并没有提高回收率。当多粘菌素浓度超过2.5微克/毫升时，对副溶血性弧菌有抑制作用。
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