一种用于快速检测海鲜中副溶血性弧菌的荧光测定法与传统方法的比较。
Comparison of a fluorogenic assay with a conventional method for rapid detection of Vibrio parahaemolyticus in seafoods.
作者信息
Venkateswaran K, Kurusu T, Satake M, Shinoda S
机构信息
Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo, Japan.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3516-20. doi: 10.1128/aem.62.9.3516-3520.1996.
A conventional method and a fluorogenic assay for the detection of Vibrio parahaemolyticus were compared. Among 29 seafood samples examined for the presence of V. parahaemolyticus, 17 samples harbored V. parahaemolyticus, and trypsinlike activity was noticed in 19 seafoods. The added fluorogenic substrate was cleaved in single samples of shrimp, turbo, and cuttlefish from which V. parahaemolyticus could not be isolated by the conventional method. Vibrio alginolyticus, in addition to V. parahaemolyticus, was found to exhibit intracellular trypsinlike activity. Trypsinlike activity in seafoods was observed after the most probable number for the initial density of V. parahaemolyticus-like organisms was found to have reached > 10(2) per g. A V. parahaemolyticus inoculum at 10(4) CFU/ml in arabinose-glucuronate medium was required to attain growth to 10(6) CFU/ml, which is the level necessary for the release of detectable amounts of fluorescent compound from the added substrate.
对检测副溶血性弧菌的传统方法和荧光测定法进行了比较。在检测副溶血性弧菌存在的29个海鲜样本中，17个样本含有副溶血性弧菌，19个海鲜样本中检测到类胰蛋白酶活性。添加的荧光底物在常规方法无法分离出副溶血性弧菌的虾、蝾螺和乌贼单个样本中被裂解。除副溶血性弧菌外，溶藻弧菌也被发现具有细胞内类胰蛋白酶活性。在类副溶血性弧菌样生物初始密度的最大可能数达到>10(2)/g后，观察到海鲜中的类胰蛋白酶活性。在阿拉伯糖 - 葡萄糖醛酸培养基中，副溶血性弧菌接种物浓度为10(4) CFU/ml时，需要生长至10(6) CFU/ml，这是从添加的底物中释放可检测量荧光化合物所需的水平。
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