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从海鲜中分离副溶血性弧菌的最佳富集时间。

Optimal enrichment time for isolation of Vibrio parahaemolyticus from seafood.

作者信息

Dupray E, Cormier M

出版信息

Appl Environ Microbiol. 1983 Nov;46(5):1234-5. doi: 10.1128/aem.46.5.1234-1235.1983.

DOI:10.1128/aem.46.5.1234-1235.1983
PMID:6651300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC239548/
Abstract

The growth of Vibrio parahaemolyticus in a liquid medium was compared with that of human fecal flora and estuarine flora. No marked differences were noted between growth at 25 and 37 degrees C for V. parahaemolyticus. However, the marine organisms were strongly inhibited when incubated at 37 degrees C. Incubation for 8 h in an enrichment broth yielded V. parahaemolyticus growth, even with a small inoculum, whereas the marine and fecal floras were inhibited. Therefore, enrichment for 8 h at 37 degrees C appears to be optimal for isolation of V. parahaemolyticus, permitting more rapid results in seafood analysis.

摘要

将副溶血性弧菌在液体培养基中的生长情况与人类粪便菌群和河口菌群进行了比较。副溶血性弧菌在25℃和37℃下生长没有明显差异。然而,海洋生物在37℃培养时受到强烈抑制。在增菌肉汤中培养8小时可使副溶血性弧菌生长,即使接种量很小,而海洋和粪便菌群则受到抑制。因此,37℃下8小时的增菌似乎是分离副溶血性弧菌的最佳条件,可在海鲜分析中更快地得到结果。

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本文引用的文献

1
Medium for isolation and differentiation of Vibrio parahaemolyticus and Vibrio alginolyticus.副溶血性弧菌和解藻酸弧菌的分离与鉴别培养基。
Appl Environ Microbiol. 1983 Jan;45(1):310-2. doi: 10.1128/aem.45.1.310-312.1983.
2
Modified selective and differential isolation medium for Vibrio parahaemolyticus.副溶血性弧菌改良选择性和鉴别性分离培养基。
Appl Microbiol. 1971 Oct;22(4):593-9. doi: 10.1128/am.22.4.593-599.1971.
3
Enrichment of Vibrio parahaemolyticus in a simple medium.副溶血性弧菌在简单培养基中的富集培养
Appl Microbiol. 1974 Jun;27(6):1124-6. doi: 10.1128/am.27.6.1124-1126.1974.
4
Ecology of Vibrio parahaemolyticus in Chesapeake Bay.切萨皮克湾副溶血性弧菌的生态学
J Bacteriol. 1973 Jan;113(1):24-32. doi: 10.1128/jb.113.1.24-32.1973.
5
Method for the detection of injured Vibrio parahaemolyticus in seafoods.海产品中受伤副溶血性弧菌的检测方法。
Appl Environ Microbiol. 1978 Jun;35(6):1121-7. doi: 10.1128/aem.35.6.1121-1127.1978.