Gholizadeh Lida, Khalili Mohammad Ali, Maleki Behnam, Vahidi Serajoddin, Agha-Rahimi Azam
Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Infertility Center, Mazandaran University of Medical Sciences, Sari, Iran.
Basic Clin Androl. 2023 Sep 7;33(1):22. doi: 10.1186/s12610-023-00198-8.
Spermatozoa retrieved from the testis and epididymis are deprived of the beneficial effects of seminal fluid. Thus applying an artificial medium with normal seminal fluid characteristics, known as artificial seminal fluid (ASF), may provide an appropriate condition for improving some sperm parameters in azoospermia. The objective was to investigate the impact of in vitro exposure of testicular and epididymal spermatozoa to ASF on sperm quality. The study was conducted on testicular (n = 20) and epididymal (n = 20) sperm specimens obtained from azoospermic men. Each sample was divided into two equal parts: Part I) for processing and incubation with Ham's F10 medium; Part II) for processing and incubation with ASF.
After 2 h incubation, testicular sperm motility was significantly higher in ASF than in Ham's F10 medium. In comparison to 0 h, mitochondrial membrane potential levels of testicular spermatozoa were significantly higher after 2 h and 24 h in ASF and after 24 h in Ham's F10 medium. Furthermore, the data indicated significantly lower rates of epididymal spermatozoa with high MMP in both media after 24 h. There were no significant differences in the DNA fragmentation index of testicular and epididymal spermatozoa between ASF and Ham's F10 medium at different time points.
The results demonstrated that in vitro incubation of testicular spermatozoa improved their motility more effectively than Ham's F10 medium in the short term (2 h), but had no effect on epididymal spermatozoa. Since the physiology of testicular spermatozoa is different from that of ejaculated spermatozoa, it seems that a special environment should be designed and used for each of them.
从睾丸和附睾中获取的精子缺乏精液的有益作用。因此，应用具有正常精液特性的人工培养基，即人工精液（ASF），可能为改善无精子症患者的某些精子参数提供适宜条件。目的是研究睾丸和附睾精子体外暴露于ASF对精子质量的影响。该研究对从无精子症男性获取的睾丸（n = 20）和附睾（n = 20）精子标本进行。每个样本分为两等份：第一部分）用于用哈姆氏F10培养基处理和孵育；第二部分）用于用ASF处理和孵育。
孵育2小时后，ASF中睾丸精子活力显著高于哈姆氏F10培养基。与0小时相比，ASF中孵育2小时和24小时后以及哈姆氏F10培养基中孵育24小时后，睾丸精子的线粒体膜电位水平显著更高。此外，数据表明24小时后两种培养基中高线粒体膜电位的附睾精子比例均显著降低。不同时间点，ASF和哈姆氏F10培养基中睾丸和附睾精子的DNA碎片化指数无显著差异。
结果表明，体外孵育睾丸精子在短期内（2小时）比哈姆氏F10培养基更有效地提高了其活力，但对附睾精子无效。由于睾丸精子的生理特性与射出精子不同，似乎应为它们分别设计和使用特殊的环境。
