Hosseini Akram, Khalili Mohammad Ali
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Transl Androl Urol. 2017 Apr;6(2):271-276. doi: 10.21037/tau.2017.03.43.
Sperm motility is the reliable parameter that roles in success of intracytoplasmic sperm injection (ICSI), especially in azoospermia. Selection of appropriate culture duration, temperature and media for enhancing the sperm motility is an important issue in assisted reproduction program. The aim was to evaluate the sperm motion characteristics after culturing of testicular sperm extraction (TESE) samples at different temperatures and time intervals.
In this prospective study, 27 TESE samples were collected from young azoospermic patients. The samples were cultured in Ham's F10+20% HAS, at different temperatures (incubation at 37 25 °C) and sperm total motility was assessed at different time intervals of 0, 24, 48 and 72 h post testicular biopsy.
culture at 25 °C changed sperm motility from 13% immediately after biopsy to 76% at 24 h, 43% at 48 h and 15% at 72 h. At 37 °C, the sperm motion feature was changed to 67% at 24 h, 38.40% at 48 h and 12.03% at 72 h. Sperm motility change at 24 h was incremental in both conditions of culturing, but significant at 25 °C (P≤0.05).
The ideal culture for testicular spermatozoa was at 25 °C after 1 day of culture, which optimized the sperm motility in azoospermic TESE samples.
精子活力是影响卵胞浆内单精子注射（ICSI）成功率的可靠参数，尤其是在无精子症患者中。选择合适的培养时间、温度和培养基以提高精子活力是辅助生殖计划中的一个重要问题。本研究旨在评估睾丸精子提取（TESE）样本在不同温度和时间间隔培养后的精子运动特征。
在这项前瞻性研究中，从年轻的无精子症患者中收集了27份TESE样本。样本在Ham's F10 + 20%人血清白蛋白（HAS）中，于不同温度（37℃和25℃孵育）下培养，并在睾丸活检后的0、24、48和72小时的不同时间间隔评估精子总活力。
在25℃培养时，精子活力从活检后立即的13%在24小时时变为76%，48小时时为43%，72小时时为15%。在37℃时，精子运动特征在24小时时变为67%，48小时时为38.40%，72小时时为12.03%。在两种培养条件下，24小时时精子活力变化均呈增加趋势，但在25℃时差异有统计学意义（P≤0.05）。
睾丸精子的理想培养条件是在25℃培养1天后，这优化了无精子症TESE样本中的精子活力。
