State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China.
Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 51015, China.
ACS Appl Mater Interfaces. 2023 Sep 20;15(37):43431-43440. doi: 10.1021/acsami.3c08326. Epub 2023 Sep 7.
Fenton-like radical processes are widely utilized to explain catalytic mechanisms of peroxidase-like nanozymes, which exhibit remarkable catalytic activity, cost-effectiveness, and stability. However, there is still a need for a comprehensive understanding of the formation, stabilization, and transformation of such radicals. Herein, a copper formate-based nanozyme (Cuf-TMB) was fabricated a pre-catalytic strategy under ambient conditions. The as-prepared nanozyme shows comparable catalytic activity (, 1.02 × 10 mM; , 3.09 × 10 s) and kinetics to those of natural peroxidase toward HO decomposition. This is attributed to the feasible oxidation by *OH species the *O intermediate, as indicated by density functional theory calculations. The key ·OH radicals were detected to be stable for over 52 days and can be released in a controlled manner during the catalytic process electron spin-resonance spectroscopy measurements. Based on the understanding, an ultrasensitive biosensing platform was constructed for the sensitive monitoring of biochemical indicators in clinic settings.
芬顿类自由基过程被广泛用于解释过氧化物酶样纳米酶的催化机制，其具有显著的催化活性、成本效益和稳定性。然而，仍然需要全面了解这些自由基的形成、稳定和转化。在此，我们采用一种基于甲酸钠的铜纳米酶（Cuf-TMB）作为一种预催化策略在环境条件下合成。所制备的纳米酶在 HO 分解方面表现出与天然过氧化物酶相当的催化活性（，1.02×10 mM；，3.09×10 s）和动力学性质。这归因于 *OH 物种对 *O 中间体的可行氧化，如密度泛函理论计算所示。关键的·OH 自由基被检测到在超过 52 天的时间内稳定，并可以通过电子自旋共振光谱测量在催化过程中以可控的方式释放。基于这一认识，构建了一个超灵敏的生物传感平台，用于在临床环境中灵敏监测生化标志物。
