Fu Yafen, Liu Tongying, Wang Xin, Wang Yanzhou, Gong Qiulin, Li Guang, Lin Qian, Zhu Siyuan
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China.
Selenium Resources Development and Utilization Center, Yichun Agricultural and Rural Bureau, Jiangxi, China.
Front Plant Sci. 2023 Aug 22;14:1217956. doi: 10.3389/fpls.2023.1217956. eCollection 2023.
Ramie is an important fiber feed dual-purpose crop in China and plays an important role in the national economy. However, ramie yield and quality can be reduced after many years of continuous cultivation. Currently, relatively little research has been conducted on rhizosphere metabolites and their pathways in continuous ramie cropping. Therefore, a healthy group (CK) and obstacle groups (XZQG, JZ, DJY, and GXD) with 8 years of continuous cultivation were selected for the study. LC-MS and GC-MS untargeted metabolomics were used to explore and analyze ramie rhizosphere metabolites and pathways. The results revealed that significant differences in the agronomic traits of ramie occurred after 8 years of continuous cultivation, with dwarfed plants and decreased yields in the obstacle groups. Metabolomic analysis identified 49 and 19 rhizosphere metabolites, including lipids, organic acids, phenols, and amino acids. In addition, four differential metabolic pathways (phenylpropanoid biosynthesis, fatty acid metabolism, amino acid metabolism, and ascorbate and aldarate metabolism) were elucidated. It was also clarified that sinapic acid, jasmonic acid, glutamine, and inositol might be the main metabolites affecting ramie continuous-cropping obstacle groups, and they were significantly correlated with ramie agronomic traits and physiological indicators. This provided important insights into the mechanisms affecting continuous ramie cropping. Accordingly, it is expected that the increase or decrease of sinapic acid, jasmonic acid, glutamine, and inositol in the soil will alleviate obstacles to continuous ramie cropping and promote the healthy development of the ramie industry in the future.
苎麻是我国重要的纤维饲用兼用作物，在国民经济中发挥着重要作用。然而，经过多年连作后，苎麻的产量和品质会下降。目前，关于苎麻连作根际代谢产物及其代谢途径的研究相对较少。因此，本研究选取了连作8年的健康组（CK）和障碍组（XZQG、JZ、DJY和GXD）。采用液相色谱-质谱联用（LC-MS）和气相色谱-质谱联用（GC-MS）非靶向代谢组学技术，对苎麻根际代谢产物及其代谢途径进行了探索和分析。结果表明，连作8年后苎麻的农艺性状出现显著差异，障碍组植株矮化，产量降低。代谢组学分析鉴定出49种和19种根际代谢产物，包括脂质、有机酸、酚类和氨基酸。此外，还阐明了四条差异代谢途径（苯丙烷生物合成、脂肪酸代谢、氨基酸代谢以及抗坏血酸和醛糖代谢）。同时明确了芥子酸、茉莉酸、谷氨酰胺和肌醇可能是影响苎麻连作障碍组的主要代谢产物，它们与苎麻农艺性状和生理指标显著相关。这为影响苎麻连作的机制提供了重要见解。据此，预计土壤中芥子酸、茉莉酸、谷氨酰胺和肌醇含量的增减，将缓解苎麻连作障碍，促进未来苎麻产业的健康发展。
