Wang Xin, Wang Yanzhou, Fu Yafen, Zhai Yang, Bai Xuehua, Liu Tongying, Li Guang, Zeng Liangbin, Zhu Siyuan
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China.
Front Plant Sci. 2024 Mar 27;15:1367862. doi: 10.3389/fpls.2024.1367862. eCollection 2024.
Beneficial bacteria that promote plant growth can shield plants from negative effects. Yet, the specific biological processes that drive the relationships between soil microbes and plant metabolism are still not fully understood. To investigate this further, we utilized a combination of microbiology and non-targeted metabolomics techniques to analyze the impact of plant growth-promoting bacteria on both the soil microbial communities and the metabolic functions within ramie () tissues. The findings indicated that the yield and traits of ramie plants are enhanced after treatment with (). These strains exhibit a range of plant growth-promoting properties, including phosphate solubilization and ammonia production. Furthermore, strain YS1 also demonstrates characteristics of IAA production. The presence of resulted in a decrease in soil bacteria diversity, resulting in significant changes in the overall structure and composition of soil bacteria communities. Metabolomics showed that significantly altered the ramie metabolite spectrum, and the differential metabolites were notably enriched ( < 0.05) in five main metabolic pathways: lipid metabolism, nucleotide metabolism, amino acid metabolism, plant secondary metabolites biosynthesis, and plant hormones biosynthesis. Seven common differential metabolites were identified. Correlation analysis showed that the microorganisms were closely related to metabolite accumulation and yield index. In the YS1 and Y4-6-1 treatment groups, the relative abundances of and were significantly positively correlated with sphingosine, 9,10,13-TriHOME, fresh weight, and root weight, indicating that these microorganisms regulate the formation of various metabolites, promoting the growth and development of ramie. Conclusively, (particularly YS1) played an important role in regulating soil microbial structure and promoting plant metabolism, growth, and development. The application of the four types of bacteria in promoting ramie growth provides a good basis for future application of biological fertilizers and bio-accelerators.
促进植物生长的有益细菌可以保护植物免受负面影响。然而，驱动土壤微生物与植物代谢之间关系的具体生物学过程仍未完全了解。为了进一步研究这一点，我们结合微生物学和非靶向代谢组学技术，分析了促生细菌对苎麻（）组织内土壤微生物群落和代谢功能的影响。研究结果表明，用（）处理后苎麻植株的产量和性状得到了提高。这些菌株具有一系列促生特性，包括解磷和产氨。此外，菌株YS1还表现出产生IAA的特性。（）的存在导致土壤细菌多样性降低，从而使土壤细菌群落的整体结构和组成发生显著变化。代谢组学表明，（）显著改变了苎麻代谢物谱，差异代谢物在五个主要代谢途径中显著富集（<0.05）：脂质代谢、核苷酸代谢、氨基酸代谢、植物次生代谢物生物合成和植物激素生物合成。鉴定出七种常见的差异代谢物。相关性分析表明，微生物与代谢物积累和产量指标密切相关。在YS1和Y4-6-1处理组中，和的相对丰度与鞘氨醇、9,10,13-三羟基十八碳烯酸、鲜重和根重显著正相关，表明这些微生物调节各种代谢物的形成，促进苎麻的生长发育。总之，（特别是YS1）在调节土壤微生物结构和促进植物代谢、生长和发育方面发挥了重要作用。这四种细菌在促进苎麻生长方面的应用为未来生物肥料和生物促进剂的应用提供了良好的基础。
