Vas Nazleen Valerie, Jain Ravindra Kumar, Ramachandran Sathish Kumar
Department of Orthodontics, Saveetha Dental College and Hospitals, Chennai, IND.
Department of Biomaterials, Saveetha Dental College and Hospitals, Chennai, IND.
Cureus. 2023 Aug 4;15(8):e42933. doi: 10.7759/cureus.42933. eCollection 2023 Aug.
Aim To prepare and characterize a 6-gingerol-incorporated chitosan biopolymer for coating on thermoformed aligners and evaluate its scratch resistance and antimicrobial activity. Material and methods In this in vitro study, 6-gingerol extract was prepared, incorporated with chitosan biopolymer into a coating solution and characterized using nuclear magnetic resonance imaging spectroscopy (NMR). Twenty thermoformed aligner samples were exposed to UV radiation for surface activation, then coated with a crosslinking agent. These were divided into four groups of five. The control group consisted of samples dip-coated in a chitosan solution for 15 minutes. The three test groups consisted of samples dip coated in a gingerol-chitosan coating solution, with each group representing the following time periods of dip coating: five, 10, and 15 minutes. The crosslinking of the coating with the aligner material was confirmed by a Fourier transform infrared spectroscopy (FTIR) test. A scratch test was carried out to evaluate the wear resistance of the coating, and the antibacterial properties of the coating were tested using a Disc Diffusion test. Results The NMR analysis confirmed the presence of 6-gingerol in the extract. The coating of 6-Gingerol on aligners was confirmed by FTIR spectroscopy. The wear resistance of aligners coated for 5 minutes, 10 minutes, and 15 minutes was 1.8 ± 0.09 N, 2.3 ± 0.021 N, and 3.06 ± 0.17 N, respectively, and the difference was statistically significant (p<0.05). The aligner coated for 15 minutes exhibited the widest zone of inhibition of up to 2.38 ± 0.44 mm against , and the difference was statistically significant (p<0.05).No antibacterial effect was found against . Conclusion A novel coating material with 6-gingerol extract incorporated in chitosan biopolymer was prepared and characterized, followed by coating on thermoformed aligners. The coating showed antibacterial activity against and both the antimicrobial activity and wear resistance increased with coating duration.
制备并表征一种负载6-姜酚的壳聚糖生物聚合物，用于热成型矫治器涂层，并评估其耐刮性和抗菌活性。材料与方法：在本体外研究中，制备6-姜酚提取物，与壳聚糖生物聚合物混合制成涂层溶液，并使用核磁共振成像光谱（NMR）进行表征。将20个热成型矫治器样品进行紫外线辐射以进行表面活化，然后用交联剂进行涂层处理。将这些样品分为四组，每组五个。对照组由在壳聚糖溶液中浸涂15分钟的样品组成。三个试验组由在姜酚-壳聚糖涂层溶液中浸涂的样品组成，每组代表以下浸涂时间段：5分钟、10分钟和15分钟。通过傅里叶变换红外光谱（FTIR）测试确认涂层与矫治器材料的交联。进行刮擦试验以评估涂层的耐磨性，并使用纸片扩散法测试涂层的抗菌性能。结果：NMR分析证实提取物中存在6-姜酚。FTIR光谱证实了6-姜酚在矫治器上的涂层。浸涂5分钟、10分钟和15分钟的矫治器的耐磨性分别为1.8±0.09 N、2.3±0.021 N和3.06±0.17 N，差异具有统计学意义（p<0.05）。浸涂15分钟的矫治器对[具体菌种1]表现出最宽的抑菌圈，可达2.38±0.44 mm，差异具有统计学意义（p<0.05）。对[具体菌种2]未发现抗菌作用。结论：制备并表征了一种新型的壳聚糖生物聚合物负载6-姜酚提取物的涂层材料，并将其涂覆在热成型矫治器上。该涂层对[具体菌种1]具有抗菌活性，且抗菌活性和耐磨性均随涂层持续时间增加。
