Potts T V, Mitra T, O'Keefe T, Zambon J J, Genco R J
Arch Microbiol. 1986 Jul;145(2):136-41. doi: 10.1007/BF00446770.
In order to assess the relationships among strains of the genera Actinobacillus and Haemophilus, DNAs from 50 strains of these genera were isolated and purified. The guanine plus cytosine (G + C) content of DNAs from strains of Haemophilus segnis and Haemophilus parainfluenzae were determined by thermal denaturation. DNA-DNA homologies were measured using labelled probes from one strain representing Haemophilus segnis (strain ATCC 10977), and two strains representing Haemophilus parainfluenzae (strains ATCC 9796 and ATCC 7901). Strains isolated as H. segnis had a G + C content of 39.0 to 42.9% and were 49-92% homologous with the ATCC 10977 DNA probe. All of the strains freshly isolated as H. parainfluenzae were 70-81% homologous with the ATCC 9796 DNA probe and had a G + C content of 34.9 to 38.3%. Strain ATCC 7901 was 11% homologous with the ATCC 9796 DNA probe, had a G + C content of 42.4%, and was 65-78% homologous to DNA from strains identified as Haemophilus aphrophilus and Haemophilus paraphrophilus. From these results we conclude that strain ATCC 7901 is a mislabelled strain of H. paraphrophilus. The results of multiple DNA-DNA hybridizations indicated that separate species designations were appropriate for H. segnis, H. parainfluenzae, Actinobacillus actinomycetemcomitans ("Haemophilus actinomycetemcomitans"), and H. aphrophilus. H. aphrophilus and H. paraphrophilus were closely related organisms and did not fulfill the generally accepted criteria for designation as separate species.
为了评估放线杆菌属和嗜血杆菌属各菌株之间的关系，从这些属的50个菌株中分离并纯化了DNA。通过热变性测定了迟缓嗜血杆菌和副流感嗜血杆菌菌株DNA的鸟嘌呤加胞嘧啶（G + C）含量。使用来自一株代表迟缓嗜血杆菌（ATCC 10977菌株）和两株代表副流感嗜血杆菌（ATCC 9796和ATCC 7901菌株）的标记探针测量DNA - DNA同源性。分离鉴定为迟缓嗜血杆菌的菌株G + C含量为39.0%至42.9%，与ATCC 10977 DNA探针的同源性为49 - 92%。所有新分离鉴定为副流感嗜血杆菌的菌株与ATCC 9796 DNA探针的同源性为70 - 81%，G + C含量为34.9%至38.3%。ATCC 7901菌株与ATCC 9796 DNA探针的同源性为11%，G + C含量为42.4%，与鉴定为嗜沫嗜血杆菌和副嗜沫嗜血杆菌的菌株DNA同源性为65 - 78%。根据这些结果，我们得出结论，ATCC 7901菌株是一株副嗜沫嗜血杆菌的错误标记菌株。多次DNA - DNA杂交结果表明，将迟缓嗜血杆菌、副流感嗜血杆菌、伴放线放线杆菌（“嗜放线嗜血杆菌”）和嗜沫嗜血杆菌分别指定为不同物种是合适的。嗜沫嗜血杆菌和副嗜沫嗜血杆菌是密切相关的生物，不符合通常公认的指定为不同物种的标准。
