针对氧化酶可变的细菌分离株的快速改良氧化酶试验。
Rapid, modified oxidase test for oxidase-variable bacterial isolates.
作者信息
Tarrand J J, Gröschel D H
出版信息
J Clin Microbiol. 1982 Oct;16(4):772-4. doi: 10.1128/jcm.16.4.772-774.1982.
Abstract
A modified oxidase reagent, 1% tetramethyl-p-phenylenediamine in dimethyl sulfoxide, proved superior to the routinely used 1% aqueous tetramethyl-p-phenylenediamine dihydrochloride in detecting weakly oxidase-positive gram-negative bacteria after 24 h of growth on agar media (40 of 40 positive versus 22 of 40 positive). The bacterial inoculum was obtained with a cotton-tipped swab instead of a loop or wooden applicator, and the reaction required less than 15 s.
摘要
一种改良的氧化酶试剂，即二甲基亚砜中1%的四甲基对苯二胺，在琼脂培养基上培养24小时后，在检测弱氧化酶阳性革兰氏阴性菌方面，被证明优于常规使用的1%盐酸四甲基对苯二胺水溶液（40株中有40株呈阳性，而40株中只有22株呈阳性）。细菌接种物是用棉签而不是接种环或木制涂抹器获取的，反应时间不到15秒。
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本文引用的文献
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Identification of Pseudomonas pyocyanea by the oxidase reaction.通过氧化酶反应鉴定绿脓杆菌。
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Role of pH in oxidase variability of Aeromonas hydrophila.pH在嗜水气单胞菌氧化酶变异性中的作用
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Evaluation of methods to detect oxidase activity in the genus Pasteurella.巴氏杆菌属中氧化酶活性检测方法的评估
J Clin Microbiol. 1980 Aug;12(2):220-5. doi: 10.1128/jcm.12.2.220-225.1980.
4
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J Clin Microbiol. 1981 Jun;13(6):1031-5. doi: 10.1128/jcm.13.6.1031-1035.1981.
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