Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Hokkaido 062-8517, Japan.
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Int J Syst Evol Microbiol. 2023 Sep;73(9). doi: 10.1099/ijsem.0.006073.
Forty-eight strains were isolated from soils and sediments in Japan. Among them, six representative strains, designated W79, W786, Red222, Red802, Red803, and Red804, were subjected to the taxonomic classification. These six strains are Gram-stain-negative, non-spore-forming, rod-shaped, and facultative anaerobic bacterium that can reduce ferric iron. Phylogenetic and phylogenomic trees based on 16S rRNA genes and multiple single-copy gene sequences showed that strains Red222, Red802, Red803, and Red804 formed a cluster with the type strains of species, but strains W79 and W786 created an independent cluster from any other type strains. The former four strains shared 97.95-99.08% similarities of 16S rRNA gene sequence with the type strains of the genus , whereas the latter two strains 94.86-95.49% similarities. The average amino acid identity of strains W79 and W786 were <63 % to any other type strains, which were below the genus delineation thresholds. Moreover, colonies of these two strains were white, while those of the other four isolated strains were reddish-yellow as well as the type strain H-5. Although the known type strains of species have been reported to be non-motile, five strains (W79, W786, Red222, Red803, and Red804) except for strain Red802 displayed motility. Furthermore, multiple genomic, phylogenetic, and phenotypic features supported the discrimination between these isolated strains. Based on the study evidence, we propose these six isolates as novel members within the ///, comprising two novel species of a novel genus, gen. nov., sp. nov., and sp. nov., and four novel species of the genus sp. nov., sp. nov., sp. nov., and sp. nov.
从日本的土壤和沉积物中分离出 48 株菌株。其中，六株代表性菌株，命名为 W79、W786、Red222、Red802、Red803 和 Red804，进行了分类学分类。这六株菌为革兰氏阴性、非孢子形成、杆状、兼性厌氧细菌，能够还原三价铁。基于 16S rRNA 基因和多个单拷贝基因序列的系统发育和系统基因组树表明，菌株 Red222、Red802、Red803 和 Red804 与属的模式菌株形成一个聚类，但菌株 W79 和 W786 与任何其他模式菌株形成一个独立的聚类。前四株菌与属的模式菌株的 16S rRNA 基因序列相似度为 97.95-99.08%，而后两株菌的相似度为 94.86-95.49%。菌株 W79 和 W786 的平均氨基酸同一性<63%至任何其他模式菌株，低于属的划分阈值。此外，这两株菌的菌落为白色，而其他四株分离株以及模式菌株 H-5 的菌落为红黄色。虽然已知的属模式菌株被报道为非运动性的，但除了菌株 Red802 之外，另外五株菌（W79、W786、Red222、Red803 和 Red804）表现出运动性。此外，多个基因组、系统发育和表型特征支持对这些分离株的区分。基于研究证据，我们提出这六株菌是 /// 的新成员，包括一个新属的两个新种，即 ，新种，和 ，新种，以及属的四个新种，即 ，新种， ，新种，和 ，新种。
