Jia Dongsheng, Liu Huan, Zhang Jian, Wan Wenqiang, Wang Zongwen, Zhang Xiaofeng, Chen Qian, Wei Taiyun
Fujian Province Key Laboratory of Plant Virology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, Fujian, People's Republic of China.
Stress Biol. 2022 Apr 15;2(1):10. doi: 10.1007/s44154-021-00032-z.
Both viruses and host cells compete for intracellular polyamines for efficient propagation. Currently, how the key polyamine-metabolizing enzymes, including ornithine decarboxylase 1 (ODC1) and its antizyme 1 (OAZ1), are activated to co-ordinate viral propagation and polyamine biosynthesis remains unknown. Here, we report that the matrix protein of rice stripe mosaic virus (RSMV), a cytorhabdovirus, directly hijacks OAZ1 to ensure the proper assembly of rigid bacilliform non-enveloped virions in leafhopper vector. Viral matrix protein effectively competes with ODC1 to bind to OAZ1, and thus, the ability of OAZ1 to target and mediate the degradation of ODC1 is significantly inhibited during viral propagation, which finally promotes polyamines production. Thus, OAZ1 and ODC1 are activated to synergistically promote viral persistent propagation and polyamine biosynthesis in viruliferous vectors. Our data suggest that it is a novel mechanism for rhabdovirus to exploit OAZ1 for facilitating viral assembly.
病毒和宿主细胞都要争夺细胞内的多胺以实现高效繁殖。目前，包括鸟氨酸脱羧酶1（ODC1）及其抗酶1（OAZ1）在内的关键多胺代谢酶是如何被激活以协调病毒繁殖和多胺生物合成的，仍不清楚。在此，我们报告水稻条纹花叶病毒（RSMV，一种细胞质弹状病毒）的基质蛋白直接劫持OAZ1，以确保在叶蝉载体中正确组装刚性杆状无包膜病毒粒子。病毒基质蛋白有效地与ODC1竞争以结合OAZ1，因此，在病毒繁殖过程中，OAZ1靶向并介导ODC1降解的能力被显著抑制，最终促进了多胺的产生。因此，OAZ1和ODC1被激活以协同促进带毒载体中的病毒持续繁殖和多胺生物合成。我们的数据表明，弹状病毒利用OAZ1促进病毒组装是一种新机制。
