Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.
Department of Veterinary Medicine, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.
Arch Virol. 2023 Sep 7;168(10):246. doi: 10.1007/s00705-023-05865-9.
In this study, 306 rectal swabs from diarrheal pigs of various ages (0-3 weeks, 3-6 weeks, and >6 weeks) were collected from 54 piggery units in different climatic zones in Haryana state, India. These samples were tested for the presence of porcine astrovirus (PAstV), porcine rotavirus group A (PRV-A), and classical swine fever virus (CSFV) by reverse transcription polymerase chain reaction (RT-PCR), and porcine circovirus 2 (PCV-2) by polymerase chain reaction (PCR). Out of the 306 samples tested, 153 (50%), 108 (35.3%), 32 (10.6%), and three (0.9%) tested positive for PAstV, PCV-2, PRV-A, and CSFV, respectively. A single infection was detected in 135 samples, while mixed infections were found in 77 samples: 70 with two viruses and seven samples with more than two. PAstV was detected most frequently (55.31%) in pigs aged 3-6 weeks. PCV-2 was more predominant in pigs aged 0-3 weeks (36.53%), whereas PRV-A was more common in pigs aged 3-6 weeks (11.3%). CSFV was observed in the age group of 0-3 weeks (1.92%). Phylogenetic analysis revealed the circulation of lineages 2 and 4 of PAstV in this region. Thus, it can be concluded that one or more than one virus is circulating in piggery units in Haryana, India.
在这项研究中，从印度哈里亚纳邦不同气候带的 54 个养猪场采集了 306 份来自不同年龄段（0-3 周、3-6 周和>6 周）腹泻猪的直肠拭子。这些样本通过反转录聚合酶链反应（RT-PCR）检测猪星状病毒（PAstV）、猪轮状病毒 A 组（PRV-A）和经典猪瘟病毒（CSFV）的存在，通过聚合酶链反应（PCR）检测猪圆环病毒 2 型（PCV-2）的存在。在测试的 306 个样本中，PAstV、PCV-2、PRV-A 和 CSFV 的阳性率分别为 153 个（50%）、108 个（35.3%）、32 个（10.6%）和 3 个（0.9%）。在 135 个样本中检测到单一感染，在 77 个样本中发现混合感染：70 个样本有两种病毒，7 个样本有两种以上病毒。PAstV 在 3-6 周龄的猪中检测到的频率最高（55.31%）。PCV-2 在 0-3 周龄的猪中更为普遍（36.53%），而 PRV-A 在 3-6 周龄的猪中更为常见（11.3%）。CSFV 在 0-3 周龄的猪中（1.92%）观察到。系统进化分析显示，PAstV 在该地区流行 2 型和 4 型谱系。因此，可以得出结论，一种或多种病毒在印度哈里亚纳邦的养猪场中传播。
