Zhao Chengxue, Chen Chen, Li Ying, Dong Shuren, Tan Kun, Tian Yuhui, Zhang Lilin, Huang Jinhai, Zhang Lei
School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai District, Tianjin, 300072, China.
Tianjin Center of Animal Disease Preventive and Control, Tianjin, China.
Arch Virol. 2019 May;164(5):1469-1473. doi: 10.1007/s00705-019-04162-8. Epub 2019 Mar 13.
Porcine astroviruses (PAstVs), are widely distributed viruses that are highly prevalent in swine herds. In this study, a novel type 4 porcine astrovirus strain (designated as PAstV4/Tianjin/2018) was identified in a fecal sample from a diarrheal piglet in Tianjin, China and its complete genomic sequence was determined by RT-PCR. Sequence analysis showed that this strain had a capsid protein with a highly variable C-terminal domain, a typical ribosomal frameshifting signal, and a conserved subgenomic promoter sequence. Recombination analysis indicated that PAstV4/Tianjin/2018 was a novel recombinant strain, and a recombination breakpoint was identified at nt position 4220 of the genome. The novel recombinant porcine astrovirus identified in China will be useful for understanding the origin, genetic diversity, and evolution of enteric viruses.
猪星状病毒（PAstVs）是广泛分布且在猪群中高度流行的病毒。在本研究中，从中国天津一头腹泻仔猪的粪便样本中鉴定出一种新型4型猪星状病毒毒株（命名为PAstV4/天津/2018），并通过逆转录聚合酶链反应（RT-PCR）测定了其完整基因组序列。序列分析表明，该毒株的衣壳蛋白具有高度可变的C末端结构域、典型的核糖体移码信号以及保守的亚基因组启动子序列。重组分析表明，PAstV4/天津/2018是一种新型重组毒株，且在基因组的第4220个核苷酸位置鉴定出一个重组断点。在中国鉴定出的这种新型重组猪星状病毒将有助于了解肠道病毒的起源、遗传多样性和进化。
