Su Mingjun, Qi Shanshan, Yang Dan, Guo Donghua, Yin Baishuang, Sun Dongbo
Laboratory for the Prevention and Control of Swine Infectious Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China.
Heilongjiang Province Cultivating Collaborative Innovation Center for the Beidahuang Modern Agricultural Industry Technology, Daqing, China.
Front Vet Sci. 2020 Aug 14;7:462. doi: 10.3389/fvets.2020.00462. eCollection 2020.
Porcine astrovirus (PAstV) is broadly distributed globally and exists as at least five distinct genotypes. PAstV, which was recently identified as an important pathogen of diarrhea in piglets, is widely distributed in China. However, few studies have investigated the coinfection and genetic characterization of PAstV in diarrheic piglets in China. In this study, 89 PAstV-positive samples were identified in 543 diarrhea samples in China from 2015 to 2018, of which 75.28% (67/89) were coinfected with three to five different porcine pathogens, while none were positive for PAstV only. Among the 543 diarrhea samples, statistical analysis showed that PAstV-induced diarrhea was potentially associated with coinfection of PEV ( < 0.01) and GARV ( < 0.01). Phylogenetic analysis showed that the 27 identified PAstV strains belong to three different genotypes and that PAstV-2 (81.48%, 22/27) was predominant in diarrheic piglets in China, followed by PAstV-4 (11.11%, 3/27) and PAasV-5 (7.41%, 2/27). Sequence analysis revealed that the 27 RdRp genes identified in this study had nucleotide homologies of 53.8-99.5%. In addition, the RdRp gene of PAstV-4 strain JL/MHK/2018/0115 harbored a unique insert of three nucleotides (GAA) as compared with other known PAstV-4 strains. Furthermore, the genotypes of PAstV varied among different geographical locations, although PAstV-2 was the most widely distributed in China. These data demonstrate that PAstV coinfection with other porcine pathogens was common and there was genetic diversity of PAstV in diarrheic piglets in China.
猪星状病毒（PAstV）在全球广泛分布，至少存在五种不同的基因型。PAstV最近被确定为仔猪腹泻的一种重要病原体，在中国广泛分布。然而，在中国，很少有研究调查腹泻仔猪中PAstV的混合感染情况和基因特征。在本研究中，2015年至2018年期间在中国的543份腹泻样本中鉴定出89份PAstV阳性样本，其中75.28%（67/89）与三到五种不同的猪病原体混合感染，而没有样本仅PAstV呈阳性。在这543份腹泻样本中，统计分析表明，PAstV引起的腹泻可能与猪细小病毒（PEV）（<0.01）和猪圆环病毒相关病毒（GARV）（<0.01）的混合感染有关。系统发育分析表明，鉴定出的27株PAstV毒株属于三种不同的基因型，其中PAstV-2（81.48%，22/27）在中国腹泻仔猪中占主导地位，其次是PAstV-4（11.11%，3/27）和PAstV-5（7.41%，2/27）。序列分析显示，本研究中鉴定出的27个RNA依赖的RNA聚合酶（RdRp）基因的核苷酸同源性为53.8%-99.5%。此外，与其他已知的PAstV-4毒株相比，PAstV-4毒株JL/MHK/2018/0115的RdRp基因有一个独特的三个核苷酸（GAA）插入。此外，尽管PAstV-2在中国分布最广，但PAstV的基因型在不同地理位置有所不同。这些数据表明，PAstV与其他猪病原体的混合感染很常见，并且中国腹泻仔猪中PAstV存在基因多样性。
